| 家猪AFLP分析体系的建立 |
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| 引用本文： | 李升康,熊远著,邓昌彦. 家猪AFLP分析体系的建立[J]. 华中农业大学学报, 2003, 22(2): 110-113 |
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| 作者姓名： | 李升康 熊远著 邓昌彦 |
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| 作者单位： | 华中农业大学农业部猪遗传改良重点实验室,武汉,430070 |
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| 基金项目： | 国家”九七三”项目 (G2 0 0 0 0 1610 5 )资助 |
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| 摘 要： | 以大白等6个猪品种为试验材料，建立了家猪扩增酶切片段长度多态性(AFLP)分析体系，对其分析过程中DNA提取、双酶切、连接、预扩增、选择性扩增、银染效果进行了检测。用E39M48引物组合构建了供试猪的AFLP指纹图谱。条带清晰可辨，扩增信号强，无背景干扰。
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| 关 键 词： | 家猪 AFLP分析体系 DNA提取 双酶切 连接 预扩增 选择性扩增 指纹图谱 |
| 修稿时间： | 2002-06-27 |
The Construction of AFLP Analysis System in Six Pig Breeds |
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| Li Shengkang Xiong Yuanzhu Deng Changyan. The Construction of AFLP Analysis System in Six Pig Breeds[J]. Journal of Huazhong Agricultural University, 2003, 22(2): 110-113 |
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| Authors: | Li Shengkang Xiong Yuanzhu Deng Changyan |
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| Abstract: | AFLP (Amplified Fragment Length Polymorphism) analysis system was established with four pig species. The results of several reactions during AFLP analysis have been detected such as DNA extraction, double enzymes restriction, adadpter ligation, preamplification and selective amplification .The AFLP fingerprinting of four pig species with primer combination E39M48 have been performed, which have 70 clear and strong amplification bands with bright background. |
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| Keywords: | AFLP DNA fingerprinting pig |
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