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苦荞麦中芦丁提取及体外抑菌活性研究
引用本文:董晓宁,赵强,杨明. 苦荞麦中芦丁提取及体外抑菌活性研究[J]. 中兽医医药杂志, 2010, 29(1): 20-22
作者姓名:董晓宁  赵强  杨明
作者单位:1. 天水师范学院生命科学与化学学院,甘肃天水,741001
2. 甘肃工业职业技术学院化学与环境工程系
3. 甘肃省畜牧兽医研究所
基金项目:甘肃省天水师范学院科研基金资助项目 
摘    要:采用乙醇回流法提取苦荞麦中的芦丁,并进行TLC检测、紫外光谱和红外光谱检测。将芦丁对两种供试菌进行体外最小抑制浓度(MIC)、最低杀菌浓度(MBC)、半数抑菌浓度(IC50)测定。结果表明,其对大肠埃希氏杆菌、金黄色葡萄球菌的MIC分别为0.40mg/mL和0.20mg/mL;MBC分别为0.50mg/mL和0.20mg/mL;IC50分别为0.2605mg/mL和0.1607mg/mL。

关 键 词:苦养麦  芦丁  提取  抑菌活性

Extraction and antibacterial activities of rutin from Fagopyrum tataricum(L.) Gaertn
DONG Xiao-ning,ZHAO Qiang,YANG Ming. Extraction and antibacterial activities of rutin from Fagopyrum tataricum(L.) Gaertn[J]. Journal of Traditional Chinese Veterinary Medicine, 2010, 29(1): 20-22
Authors:DONG Xiao-ning  ZHAO Qiang  YANG Ming
Affiliation:DONG Xiao-ning (College of Life Science , Chemistry,Tianshui Normal University,Tianshui Gansu 741001,China)
Abstract:Rutin were extracted from Fagop rum tataricum(L.) Gaertn. and tested through TLC,ultraviolet spectrum and infrared spectrum. Then its minimal inhibitory concentration(MIC),minimal bactericidal concentration (MBC) and half inhibitory concentration(IC50) on against Escherichia coli and Staphylococcus aureus were measured by means of bacteriostasis tests in vitro. Results showed that MIC of the rutin against Escherichia coli and Staphylococcus aureus was 0.40 and 0.20 mg/mL,MBC was 0.50 and 0.20 mg/mL,IC50 was...
Keywords:Fagopy rum tataricum(L.) Gaertn.  rutin  extraction  antimicrobial activity  
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