| 盆栽小菊高效遗传转化体系的建立 |
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| 引用本文： | 于淼,刘兆磊,陈素梅,陈发棣,章镇.盆栽小菊高效遗传转化体系的建立[J].江苏农业学报,2009,25(3). |
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| 作者姓名： | 于淼 刘兆磊 陈素梅 陈发棣 章镇 |
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| 作者单位： | 南京农业大学园艺学院,江苏,南京,210095 |
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| 基金项目： | 江苏省高技术研究项目 |
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| 摘 要： | 采用根癌农杆菌介导的叶圆片遗传转化方法,建立了盆栽小菊稳定而高效的遗传转化体系.试验结果表明:黑暗条件下预培养2d,根癌农杆菌A600约0.5、侵染10 min,共培养3 d,延迟培养5 d能获得较高的转化率;10～15 mg/L的潮霉素具有很好的筛选效果.获得的抗性芽再生植株经PCR检测,初步证实外源基因已转入受体植物的部分株系中.
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| 关 键 词： | 菊花 根癌农杆菌 遗传转化 |
Establishment of Highly Efficient Transfermation System for Small Flowered Pot Chrysanthemum |
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| YU Miao,LIU Zhao-lei,CHEN Su-mei,CHEN Fa-di,ZHANG Zhen.Establishment of Highly Efficient Transfermation System for Small Flowered Pot Chrysanthemum[J].Jiangsu Journal of Agricultural Sciences,2009,25(3). |
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| Authors: | YU Miao LIU Zhao-lei CHEN Su-mei CHEN Fa-di ZHANG Zhen |
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| Institution: | College of Horticulture;Nanjing Agricultural University;Nanjing 210095;China |
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| Abstract: | A stable and efficient genetic transformation system for small flowered pot chrysanthemum via Agrobacterium-mediated leaf disc transformation was established.High transformation frequency was obtained when the leaves were pre-cultured for 2 d in the dark,then inoculated with Agrobacterium tumefaciens at A600 about 0.5 for 10 min,co-cultivated for 3 d and hygromycin free culture prolonged for 5 d.The optimal selective concentration of hygromycin was 10-15 mg/L.Several hygromycin resistant transformants were ... |
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| Keywords: | Dendranthema morifolium Agrobacterium tumefacien genetic transformation |
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