| 低温对山定子和平邑甜茶幼苗根系氮代谢酶及游离氨基酸的影响 |
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| 引用本文： | 王英,吕德国,秦嗣军,张玉龙,马怀宇,刘国成,孟倩. 低温对山定子和平邑甜茶幼苗根系氮代谢酶及游离氨基酸的影响[J]. 园艺学报, 2010, 37(2): 179-184 |
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| 作者姓名： | 王英 吕德国 秦嗣军 张玉龙 马怀宇 刘国成 孟倩 |
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| 作者单位： | 1. 沈阳农业大学园艺学院,沈阳,110161
2. 沈阳农业大学土地与环境学院,沈阳,110161 |
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| 基金项目： | 现代农业产业技术体系建设专项项目，公益性行业(农业)科研专项项目，辽宁省自然儿科学基金项目，辽宁省农业攻关计划项目 |
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| 摘 要： | 以苹果砧木山定子(Malus baccata Borkh.)和平邑甜茶(Malus hupehensis Rehd.)实生苗为试材,研究低温(昼10℃/夜2℃)对根系氮代谢关键酶活性和氨基酸含量的影响。结果表明:低温诱导山定子和平邑甜茶根系谷氨酰胺合成酶(GS)活性,与常温对照相比两砧木活性分别增加了119%和317%。低温同时降低了谷氨酸合酶(NADH-GOGAT)和谷氨酸脱氢酶(NADH-GDH)活性,两砧木NADH-GOGAT活性分别为常温对照的6.11%和48.43%,NADH-GDH活性分别为常温对照的47.07%和33.84%。低温对平邑甜茶GS活性和NADH-GDH活性的影响大于山定子,说明平邑甜茶对低温胁迫较敏感;山定子根系NADH-GOGAT活性受低温影响较大,而低温对平邑甜茶根系NADH-GOGAT活性没有明显影响。GS/GDH比值分析表明,低温前后根系中铵同化途径均以GS/GOGAT为主,但低温使GS/GOGAT途径在根系中的作用明显加强,尤其对平邑甜茶的效果更明显。低温增加了山定子和平邑甜茶根系游离氨基酸总量,山定子和平邑甜茶分别比对照增加了78.34%和89.79%,平邑甜茶游离氨基酸含量高于山定子。低温处理前根系中游离氨基酸以苏氨酸、酪氨酸、天冬氨酸和谷氨酸为主,低温处理后以苏氨酸、精氨酸、天冬氨酸和谷氨酸为主,低温显著提高了根系精氨酸的含量。
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| 关 键 词： | 山定子 平邑甜茶 谷氨酰胺合成酶 谷氨酸合酶 谷氨酸脱氢酶 游离氨基 |
Effects of Low-temperature on Enzymes Activities of Nitrogen Metabolism and Free Amino Acids Contents in Root of Malus baccata Borkh.and Malus hupehensis Rehd.Seedlings |
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| WANG Ying,Lü De-guo,QIN Si-jun,ZHANG Yu-long,MA Huai-yu,LIU Guo-cheng,MENG Qian. Effects of Low-temperature on Enzymes Activities of Nitrogen Metabolism and Free Amino Acids Contents in Root of Malus baccata Borkh.and Malus hupehensis Rehd.Seedlings[J]. Acta Horticulturae Sinica, 2010, 37(2): 179-184 |
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| Authors: | WANG Ying Lü De-guo QIN Si-jun ZHANG Yu-long MA Huai-yu LIU Guo-cheng MENG Qian |
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| Affiliation: | (Horticulture College, Shenyang Agricultural University, Shenyang 110161, China;College of Land and Environmental Science, Shenyang Agricultural University, Shenyang 110161, China) |
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| Abstract: | The enzymes activities of nitrogen metabolism and free amino acids were studied under low temperature in Malus baccata Borkh.and M.hupehensis Rehd.The results indicated that at low temperature,glutamine synthetase(GS) activity were increased obviously by 119% and 317% respectively in M.baccata Borkh.and M.hupehensis Rehd.But low temperature decreased glutamate synthase (NADH-GOGAT) and glutamate dehydrogenase (NADH-GDH) activity.In M.baccata Borkh.and M.hupehensis Rehd.NADH-GOGAT activity decreased to 6.11%... |
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| Keywords: | Malus baccata Borkh. M.hupehensis Rehd. glutamine synthetase glutamate synthase glutamate dehydrogenase free amino acids |
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