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苜蓿提取液抑菌活性研究
引用本文:卢小康,蒲万霞,张咏梅,李宝兰,曹致忠,刘英. 苜蓿提取液抑菌活性研究[J]. 安徽农业科学, 2008, 36(31)
作者姓名:卢小康  蒲万霞  张咏梅  李宝兰  曹致忠  刘英
作者单位:甘肃农业大学,甘肃兰州,730000;中国农业科学院兰州畜牧与兽药研究所,甘肃兰州,730000
基金项目:国家科技支撑计划  
摘    要:[目的]研究苜蓿提取液对4种常见菌绿脓杆菌、停乳链球菌、大肠杆菌和金黄色葡萄球菌的抑菌活性。[方法]采用二倍稀释法和血平板鉴定相结合的方法测定其最低抑菌浓度(MIC)。[结果]苜蓿提取液对绿脓杆菌和停乳链球菌的MIC分别为0.674和1.347mg/ml;对大肠杆菌和金黄色葡萄球菌的MIC均为2.695 mg/ml。[结论]苜蓿提取液对绿脓杆菌和停乳链球菌有较好的抑菌活性,对大肠杆菌和金黄色葡萄球菌的抑菌效果一般。

关 键 词:苜蓿提取液  二倍稀释法  血平板鉴定  最低抑菌浓度

Study on the Antibacterial Activity of Alfalfa Extract
Abstract:[Objective] The research aimed to study the antibacterial activity of alfalfa extract on 4 kinds of common bacteria including Pseudomonas aeruginosa, Streptococcus dysgalactiae,Escherichia coil and Staphyloccocus aureus.[Method] By using double dilution method and blood plate identification,the minimal inhibitory concentrations(MIC) were determined.[Result] MIC of alfalfa extract to P.aeruginosa and S.dysgalactiae were 0.674 and 1.347 mg/ml respectively.MIC of alfalfa extract to E.coil and S.aureus were both 2.695 mg/ml.[Conclusion] Alfalfa extract had better antibacterial activity to P.aeruginosa and S. dysgalactiae.But the antibacterial effects on E.coil and S.aureus were ordinary.
Keywords:Alfalfa extract  Double dilution method  Blood plate identification  Minimal inhibitory concentration
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