苜蓿提取液抑菌活性研究 |
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引用本文: | 卢小康,蒲万霞,张咏梅,李宝兰,曹致忠,刘英. 苜蓿提取液抑菌活性研究[J]. 安徽农业科学, 2008, 36(31) |
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作者姓名: | 卢小康 蒲万霞 张咏梅 李宝兰 曹致忠 刘英 |
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作者单位: | 甘肃农业大学,甘肃兰州,730000;中国农业科学院兰州畜牧与兽药研究所,甘肃兰州,730000 |
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基金项目: | 国家科技支撑计划 |
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摘 要: | [目的]研究苜蓿提取液对4种常见菌绿脓杆菌、停乳链球菌、大肠杆菌和金黄色葡萄球菌的抑菌活性。[方法]采用二倍稀释法和血平板鉴定相结合的方法测定其最低抑菌浓度(MIC)。[结果]苜蓿提取液对绿脓杆菌和停乳链球菌的MIC分别为0.674和1.347mg/ml;对大肠杆菌和金黄色葡萄球菌的MIC均为2.695 mg/ml。[结论]苜蓿提取液对绿脓杆菌和停乳链球菌有较好的抑菌活性,对大肠杆菌和金黄色葡萄球菌的抑菌效果一般。
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关 键 词: | 苜蓿提取液 二倍稀释法 血平板鉴定 最低抑菌浓度 |
Study on the Antibacterial Activity of Alfalfa Extract |
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Abstract: | [Objective] The research aimed to study the antibacterial activity of alfalfa extract on 4 kinds of common bacteria including Pseudomonas aeruginosa, Streptococcus dysgalactiae,Escherichia coil and Staphyloccocus aureus.[Method] By using double dilution method and blood plate identification,the minimal inhibitory concentrations(MIC) were determined.[Result] MIC of alfalfa extract to P.aeruginosa and S.dysgalactiae were 0.674 and 1.347 mg/ml respectively.MIC of alfalfa extract to E.coil and S.aureus were both 2.695 mg/ml.[Conclusion] Alfalfa extract had better antibacterial activity to P.aeruginosa and S. dysgalactiae.But the antibacterial effects on E.coil and S.aureus were ordinary. |
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Keywords: | Alfalfa extract Double dilution method Blood plate identification Minimal inhibitory concentration |
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