Segregation distortion in doubled haploid lines of barley (Hordeum vulgare L.) detected by simple sequence repeat (SSR) markers |
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| Authors: | Haitham Sayed Hamed Kayyal Luke Ramsey Salvatore Ceccarelli Michael Baum |
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| Institution: | (1) ICARDA, The International Center for Agricultural Research in the Dry Areas (, P.O. Box 4566, Aleppo, Syria;(2) Faculty of Agriculture, Damascus University, Damascus, Syria;(3) Scottish Crop Research Institute, Dundee, Scotland |
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| Abstract: | A total of 147 simple sequence repeat (SSR) markers (including86 barley and 61 wheat microsatellite markers) were tested for
their segregation in a doubled haploid (DH) and an F2 population of barley. The DH population consisted of 71 doubled haploid lines, developed from F1 plants of a cross between Tadmor and WI2291using isolated microspore culture technique. A genetic linkage map consisting
of 43 microsatellite markers was constructed using the DH population. Particularly on chromosome 4H microsatellite markers
showed distorted segregation ratios. Segregation of DH lines based on molecular markers were compared with segregation of
92 F2 lines from the same cross. The proportion of loci deviating from the expected monogenic segregation ratios in the DH population
was significantly higher (19/43loci, 44%) than in the F2 population (7/43 loci, 16%). The deviation was biased towards the WI2291 parent alleles. In line with this observation, WI2291
was found to perform better than Tadmor in regenerating green plantlets with the isolated microspore-culture technique.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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| Keywords: | barley (Hordeum vulgare) doubled haploid (DH) microspore culture (MC) segregation distortion and simple sequencerepeat (SSR) |
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