| 正交设计优选薏苡茎多糖的酶提取工艺 |
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| 引用本文： | 郭立强,黄礼德,张照平,潘廷啟,文全泰,颜祖弟,黄锁义. 正交设计优选薏苡茎多糖的酶提取工艺[J]. 安徽农业科学, 2012, 40(21): 10876-10877 |
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| 作者姓名： | 郭立强 黄礼德 张照平 潘廷啟 文全泰 颜祖弟 黄锁义 |
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| 作者单位： | 右江民族医学院,广西百色,533000;右江民族医学院,广西百色,533000;右江民族医学院,广西百色,533000;右江民族医学院,广西百色,533000;右江民族医学院,广西百色,533000;右江民族医学院,广西百色,533000;右江民族医学院,广西百色,533000 |
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| 基金项目： | 广西自然科学基金资助课题，广西中医药科技专项资助课题，广西教育厅课题，广西中医药管理局课题，广西医学科学实验中心开放基金专项资助项目，右江民族医学院民族医药协会资助项目 |
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| 摘 要： | [目的]确定薏苡茎多糖的最佳酶提取工艺。[方法]利用纤维素酶从薏苡茎中提取多糖,采用正交试验方法进行条件优选,并用分光光度法对提取物进行多糖的含量测定。[结果]检测波长为490 nm;葡萄糖含量在0.010～0.100 mg/ml范围内与吸光度呈良好的线性关系(R=0.999 8);最佳酶提取工艺为:pH值为5,酶用量为6.0 ml,反应温度为40℃,酶解时间为150 min;在此条件下,薏苡茎多糖的平均提取率为19.62%,RSD为0.21%。[结论]优选出的酶提取工艺合理,经济,可行。
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| 关 键 词： | 薏苡茎 多糖 纤维素酶 正交设计 |
Optimization of Enzyme Extracting Process of Polysaccharides from Coix lachrymal-jobi L.Stems by Orthogonal Design |
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| Affiliation: | GUO Li-qiang et al(Youjiang Medical College for Nationalities,Baise,Guangxi 533000) |
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| Abstract: | [Objective] To determine the optimal enzyme extracting process of polysaccharides from Coix lachrymal-jobi L.stems.[Method] The cellulase extraction process of polysaccharides from C.lachrymal-jobi stems was optimized by means of orthogonal test design.And the content of polysaccharides in extracts was determined by spectrophotometry.[Result] The wavelength of the maximum absorption was 490 nm.Glucose concentration showed a good linear relationship with absorbance at a range of 0.010 to 0.100 mg/ml(R=0.999 8).The optimal technological conditions for the extraction of polysaccharides were as follows: pH value was 5,amount of enzyme was 6 ml,the reaction temperature was 40 ℃,and enzymatic hydrolysis time was 150 min.Under these conditions,the mean extraction rate of polysaccharides was 19.62% with RSD of 0.21%.[Conclusion] The enzyme extracting procedure is reasonable,economical and feasible. |
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| Keywords: | Coix lachrymal-jobi L.stems Polysaccharides Cellulase Orthogonal design |
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