意大利蜜蜂碳酸酐酶相关蛋白X的基因克隆及序列分析

目的]对意大利蜜蜂(Apis mellifera)碳酸酐酶相关蛋白(CARPs)X的基因进行克隆,并对其序列进行分析。方法]通过RT-PCR扩增得到CARP X基因的cDNA序列,同时对序列进行生物信息学分析。结果]意大利蜜蜂CARP X基因的cDNA全长972 bp,编码324个氨基酸残基,推导的氨基酸序列具有1个信号肽、2个跨膜区;预测其分子量和等电点分别为37.1 kD和7.458;该蛋白质与小蜜蜂(Apis florea)、熊蜂(Bombus impatiens)、Bombus terrestris、金小蜂(Nasonia vitripennis)、豌豆蚜虫(Acyrthosiphon pisum)的CARP X有很近的亲缘关系;聚类分析显示在昆虫体内可能具有2种不同类型的CARP X。结论]该研究为CARPs蛋白家族的研究提供了可靠的理论基础。

Cloning and Sequence Analysis of Carbonic Anhydrase-Related Protein 10-like in Apis mellifera

Objective] This study aimed to clone and analyze the gene sequence encoding carbonic anhydrase-related protein 10-like from Apis mellifera.Method] The cDNA sequence of CARP X gene was cloned through RT-PCR method,and then analyzed with bioinformatical method.Result] The full cDNA sequence of CARP X was 972 bp long,encoding 324 amino acid residues,including a signal peptide and two transmembrane domains.The predicted molecular mass was 37.1 kDa and the predicted isoelectric point was 7.458.The CARP X from A.mellifera showed the close relationship with proteins from Apis florae,Bombus impatiens,Bombus terrestris,Nasonia vitripennis and Acyrthosiphon pisum.The insect CARP X family may contain two subfamilies.Conclusion] The results provided the basis for studying CARPs family.