樱桃叶多糖的提取、鉴别及定量测定 |
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引用本文: | 刘德胜,颜玲,韩景田. 樱桃叶多糖的提取、鉴别及定量测定[J]. 安徽农业科学, 2012, 40(21): 10861-10862,10872 |
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作者姓名: | 刘德胜 颜玲 韩景田 |
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作者单位: | 滨州医学院药学院,山东烟台,264003;滨州医学院药学院,山东烟台,264003;滨州医学院药学院,山东烟台,264003 |
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摘 要: | [目的]提取樱桃叶多糖,并对其进行定性鉴别和含量测定。[方法]先将樱桃叶经石油醚和乙醇脱脂、脱杂后,再采用水提醇沉法提取樱桃叶粗多糖(PPP),然后以Sevag法除去粗多糖中的蛋白,并用Molish试验(α-萘酚试验)、葸酮-硫酸法试验、Fehling试验与红外光谱分析、淀粉的碘试验对其进行定性鉴定;以硫酸-蒽酮法测定樱桃叶多糖的含量。[结果]经过鉴别可知,所提灰白色物质为多糖;经测定,其含量为29.7 mg/g。[结论]该方法简单,灵敏度高,重现性好,结果准确。
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关 键 词: | 樱桃叶 多糖 提取 鉴定 硫酸-蒽酮法 |
Extraction, Identification and Content Determination of Polysaccharides from the Leaves of Prunus pseudocerasus Lindl |
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Affiliation: | LIU De-sheng et al(College of Pharmacy,Binzhou Medical College,Yantai,Shandong 264003) |
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Abstract: | [Objective] To study the extraction,qualitative identification and quantitative assay of polysaccharides from the leaves of Prunus pseudocerasus Lindl.(PPP),so as to provide reference for its comprehensive application.[Method] Polysaccharides were extracted with the method of water exaction and alcohol precipitation after the leaves were defatted with 95% EtOH and petroleum ether.Then,the protein in PPP was removed by Sevag method.A series of experiments were used to identify the PPP,including Molish test(α-naphthol test),anthrone-sulfuric acid method test,fehling test,fourier transform infrared spectrometry(FT-IR),and iodine test.The content of PPP was determined by anthrone-sulphuric acid colrimetry method.[Results] P.pseudocerasus polysaccharides were grey white in color.The content of polysaccharides in P.pseudocerasus leaves was 29.7 mg/g.[Conclusion] The method is simple,sensitive,reproducible,and reliable. |
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Keywords: | Prunus pseudocerasus Lindl. Polysaccharides Extraction Identification Anthrone-sulfuric acid method |
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