| 相似穿孔线虫伴生细菌群落多样性研究 |
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| 引用本文： | 郑芬,符美英,赵志祥,陈绵才.相似穿孔线虫伴生细菌群落多样性研究[J].安徽农业科学,2012,40(22):11274-11277. |
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| 作者姓名： | 郑芬 符美英 赵志祥 陈绵才 |
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| 作者单位： | 海南大学环境与植物保护学院,海南海口570228;海南省农业科学院农业环境与植物保护研究所,海南省植物病虫害综合防控重点实验室,海南海口571100;海南省农业科学院农业环境与植物保护研究所,海南省植物病虫害综合防控重点实验室,海南海口571100 |
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| 基金项目： | 公益性行业(农业)科研专项 |
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| 摘 要： | 目的]揭示相似穿孔线虫伴生细菌群落物种丰度及其细菌多样性。方法]通过构建细菌16S rDNA克隆文库,对阳性克隆进行ARDRA分析和构建系统发育树。结果]依据97%序列相似性划分OTU,构建的细菌16S rDNA文库包含13个OTU,分别属于Alpha-proteobacteria、Betaproteobacteria和Gammaproteobacteria,其中优势菌群为Betaproteobacteria,特别是Burkholderia为优势细菌。结论]相似穿孔线虫的伴生细菌多样性较高,这些细菌可能对相似穿孔线虫具有一定的生态意义。
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| 关 键 词： | 相似穿孔线虫 伴生细菌 ARDRA 系统进化分析 |
Community Diversity of Associated Bacteria of Radopholus similis |
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| Institution: | ZHENG Fen et al(College of Environment and Plant Protection,Hainan University,Haikou,Hainan 570228) |
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| Abstract: | Objective] The paper was to reveal the species abundance of associated bacterial community,and the diversity of its bacteria.Method] Through the construction of 16S rDNA cloning library of bacteria,the positive clone was carried out ARDRA analysis,and the phylogenetic tree was constructed.Result] OUT(Operational Taxonomic Units) was classified according to sequences similarity of 97%,the constructed 16S rDNA library of bacteria contained 13OTUs,belonging to Alphaproteobacteria,Betaproteobacteria and Gammaproteobacteria,respectively.The dominant species were Betaproteobacteria,especially Burkholderia was the dominant bacterium.Conclusion] The diversity of associated bacteria of R.similis was high,and these bacteria may have certain ecological significance to R.similis. |
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| Keywords: | Radopholus similis Associated bacteria ARDRA Phylogenetic analysis |
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