| GmSARK启动子驱动IPT基因在拟南芥中的表达研究 |
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| 引用本文： | 霍巍巍,闫璇玲,孙晓文,李海燕. GmSARK启动子驱动IPT基因在拟南芥中的表达研究[J]. 安徽农业科学, 2012, 40(22): 11147-11150 |
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| 作者姓名： | 霍巍巍 闫璇玲 孙晓文 李海燕 |
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| 作者单位： | 吉林农业大学生命科学学院,吉林长春,130118;长春市农民职业教育中心,吉林长春,130052;吉林省镇赉县芦苇湿地管理局,吉林镇赉,137300;吉林农业大学生命科学学院,吉林长春130118;吉林农业大学生物反应器与药物开发教育部工程研究中心,吉林长春130118 |
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| 基金项目： | 国家转基因生物新品种培育重大专项，教育部新世纪优秀人才计划项目，吉林省优秀创新团队项目，长春市科技局项目 |
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| 摘 要： | [目的]对GmSARK启动子驱动IPT基因在拟南芥中的表达进行研究。[方法]分别克隆IPT基因及GmSARK基因启动子,构建它们的植物表达载体并进行拟南芥的转化,利用PPT(phosphinothricin)除草剂筛选,检测T1代转基因植株;对T1代转基因植株进行黑暗避光和干旱处理后进行半定量RT-PCR分析。[结果]成功克隆得到了IPT基因及GmSARK基因,并构建了它们的p3301-GmSARK-IPT植物表达载体;对T1代转基因植株的RT-PCR表明,目的基因mRNA水平上有所表达。[结论]为进一步研究GmSARK启动子驱动的IPT基因在抗逆中的作用奠定了基础。
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| 关 键 词： | IPT基因 GmSARK启动子 拟南芥 |
Heterologous Expression of Isopentenyl Transferase(Ipt) Gene Directed by Senescence Associated Receptor Protein Kinase(SARK) Promoter in Arabidopsis |
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| Affiliation: | HUO Wei-wei et al(College of Life Sciences,Jilin Agricultural University,Changchun,Jilin 130118) |
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| Abstract: | [Objective] This study aimed to investigate the expression of IPT gene directed by GmSARK promoter in Arabidopsis.[Method] IPT gene and GmSARK promoter were respectively cloned to construct plant expression vector and transform Arabidopsis.T1 transgenic plant lines were detected by PPT(phosphinothricin) herbicide selection and treated under drought and dark conditions for semi-quantitive RT-PCR analysis.[Result] GmSARK and IPT fusion gene was successfully cloned and the plant expression vector p3301-GmSARK-IPT was constructed.RT-PCR analysis showed that the target gene was expressed in T1 transgenic plant lines at the mRNA level.[Conclusion] This study laid the foundation for further investigating the roles of IPT gene directed by GmSARK promoter in plant stress resistance. |
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| Keywords: | IPT gene GmSARK promoter Arabidopsis |
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