| 猪繁殖与呼吸综合征病毒非结构蛋白2的生物信息学分析 |
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| 引用本文： | 孙荡,高歌,周胜,鲍梦雅,茅翔.猪繁殖与呼吸综合征病毒非结构蛋白2的生物信息学分析[J].安徽农业科学,2012,40(21):10903-10905. |
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| 作者姓名： | 孙荡 高歌 周胜 鲍梦雅 茅翔 |
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| 作者单位： | 南京农业大学动物医学院,江苏南京,210095;南京农业大学动物医学院,江苏南京,210095;南京农业大学动物医学院,江苏南京,210095;南京农业大学动物医学院,江苏南京,210095;南京农业大学动物医学院,江苏南京,210095 |
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| 基金项目： | 南京农业大学大学生创新项目(SRT项目) |
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| 摘 要： | 目的]为临床上构建PRRSV的拮抗性小肽及相关疫苗设计提供理论参考。方法]对NCBI上国内分离的PRRSV毒株(FJ175688.1)Nsp2的氨基酸序列进行生物信息学方法分析,并对其组分、理化性质、跨膜结构域、二级结构、糖基化位点、磷酸化位点和B细胞抗原表位进行预测。结果]Nsp2含有7段的跨膜区域,二级结构中自由卷曲含量最高(57.64%),同时含有4个糖基化位点和76个磷酸化位点。综合分析表明,Nsp2具有大量的抗原决定簇。结论]通过对Nsp2的生物信息学分析,可为PRRSV疫苗设计提供理论基础。
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| 关 键 词： | PRRSV 非结构蛋白2 生物信息学 结构与功能 |
Bioinformatics Analysis of Non-structural Protein 2 of PRRSV |
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| Institution: | SUN Dang et al(College of Veterinary Medicine,Nanjing Agricultural University,Nanjing,Jiangsu 210095) |
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| Abstract: | Objective] The research aimed to provide theoretical basis for constructing the antagonistic small-peptide and designing the related vaccine in clinic.Method] The bioinformatics analysis was made on amino acid sequences of non-structural protein 2(Nsp 2) of PRRSV strain isolated in China on NCBI website.And its composition,physical and chemical characters,transmembrane domain,secondary structure,glycosylation site,phosphorylation site and B cell epitope were predicted.Result] Nsp2 contained 7 transmembrane regions,4 glycosylation sites and 76 phosphorylation sites.The content of random coil in the secondary structure was the highest(57.64%).The comprehensive analysis showed that Nsp2 had a great lot of epitopes.Conclusion] The bioinformatic analysis of Nsp2 could provide theoretical basis for the vaccine design of PRRSV. |
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| Keywords: | PRRSV Nsp2 Bioinformatics Structure and function |
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