葡萄开放式组织培养外植体系的建立 |
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作者姓名: | 崔刚 单文修 秦旭 孙仲序 |
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作者单位: | 山东农业大学园艺科学与工程学院 泰安271018
(崔刚),山东省农业厅 济南250014
(单文修,秦旭),山东农业大学园艺科学与工程学院 泰安271018(孙仲序) |
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摘 要: | 为了在开放组培条件下获得葡萄高效外植体系,对抑生素的使用浓度、浸泡时间和培养浓度进行了大量比较试验。试验结果表明:抑生素浸泡浓度为4%,浸泡时间为6h,培养浓度为0.2%时,葡萄外植体的成功率能够达到95%以上,所获外植体分化正常。
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关 键 词: | 葡萄 开放组培 外植体 抑生素 |
Establishment of Grape Explant in Open Tissue Culture |
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Authors: | Cui Gang Shan Wenxiu Qin Xu Sun Zhongxu |
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Abstract: | To obtain high efficient grape explant system in open-tissue-culture,a lot of compared experiments were made on plantiotics concentration ,soaked time and concentration in medium. Experimental results showed that soaked in 4%for 6h and inserted in 0.2% medium, the succeed percentage of grape explant could arrived 95 % above, and the differentiation was normal. |
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Keywords: | Grape Open-tissue-culture Explant Plantiotics |
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