基于SSR标记和表型性状构建苦瓜核心种质的研究

利用苦瓜21个SSR标记和9个表型性状数据,对地理来源多样的184份苦瓜种质资源进行核心种质构建.结合基因型数据的M策略抽样法和表型数据的随机抽样法,共抽取出34份苦瓜种质资源作为核心种质.34份苦瓜核心种质保留了原始种质93.46％的等位基因和大部分地理来源.对核心种质的SSR基因型代表性进行评价,核心种质和原始种质...

Studies on Construction of Bitter Gourd Core Collection Based on SSR Markers and Phenotypic Traits

Taking advantage of 21 bitter gourd SSR markers and 9 phenotypic traits,this paper constructed core collection for 184 bitter gourd germplasm resources from various geographical origins;and extracted 34 accessions as core collection by combining M strategy sampling method of genotype data and random sampling method of phenotype data.These 34 bitter gourd core collection had reserved 93.46%of the alleles and most of the geographical origins of the initial germplasm.The paper also carried out evaluation on the representativeness of SSR genotype.The results showed that there were no significant differences in the 6 genetic diversity parameters between the core collection and the initial collection.They were the number of alleles(N_a),effective number of alleles(N_e),Shannon’s Information index(I),heterozygosity(H),gene diversity(GD)and polymorphism information content(PIC).The evaluation on the phenotypic representativeness of core collection showed that the mean difference percentage(MD%)was 0,and coincidence rate(CR%)was 88.30%.The above results indicated that the 34 core collection of bitter gourd could represent the diversity of initial collection in terms of genotype and phenotype,meeting the requirement of core collection construction.