磷对条斑紫菜单细胞固体培养的影响

酶解条斑紫菜（Porphyra yezoensis）得到单细胞，培养于添加不同浓度H2PO4^-的0．7％琼胶培养基上。观察磷浓度变化对细胞分化发育速度、细胞团和幼苗的比例、幼苗生长和单孢子放散等的影响，探讨条斑紫菜单细胞固体培养的适宜磷浓度。结果显示，在磷质量浓度10mg／L、20mg／L时，细胞的发育速度和幼苗的生长速度都比较快，能够形成正常大苗。培育14d幼苗即大量放散单孢子。在添加4mg／L磷营养盐的培养基上，幼苗生长慢，培育22d能够形成少量大苗，未见放散单孢子。当磷质量浓度低于2mg／L或高于40mg／L均不能形成正常大苗。

Effects of phosphorus on cell development of Porphyra yezoensis in solid culture

To culture cells and tissues on medium with certain percentage of agar is called solid culture.Since cultivars on solid medium can be located in fixed position,the development process of a certain cell can be easily investigated and the interference between cells can be highly decreased. Therefore solid culture is applied in the present study to understand the development and differentiation pathways of vegetative cells of Porphyra yezoensis.Vegetative cells were cultured on solid media(0.7% agar) enriched with varied concentration of phosphorus(all enriched with 40?mg/L nitrogen).Division and development of vegetative cells,growth and development of cell seedlings were studied.The results show that: 1) At the concentrations of 10?mg/L and 20?mg/L,cells divide and develop faster,and a lot of large and normal cell seedlings are formed after 22 days of culture.Cell seedlings can release monospores after 2 weeks of culture.2) At the concentration of 4?mg/L,cell seedings grow slowly.Smaller quantities of large normal seedlings can be formed after 22 days of culture.No monospores are released during the experimental period.3) No large and normal seedling formed when phosphorus concentrations were lower than 2?mg/L or higher than(40?mg/L.)