Cryopreservation of Delphinium pollen at –30 °C |
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Authors: | Kazushige Honda Hitoshi Watanabe Kiyoshi Tsutsui |
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Affiliation: | (1) Department of Forestry & Landscape Architecture, Hokkaido College, Senshu University, Bibai 079-0197, Japan;(2) Faculty of Horticulture, Chiba University, Matsudo 648, Chiba 271-8510, Japan;(3) Research Section, Hokkai Sankyo Co., Ltd., Kitanosato, Kitahiroshima 061-1111, Japan |
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Abstract: | Pollen of garden varieties and species of Delphinium that was cryopreserved at –30 °C after 3 hours of air drying and storage on silica gel, had high germination and pollen tube growth in vitroeven after 180-day storage. On the other hand, pollen stored at 25 °C showed a marked decrease in the germination rate within 10 days. The best in vitro germination of Delphinium pollen was on a 1% water agar containing 15% sucrose and 0.005 to 0.01% boric acid and at 15 to 20 °C. Field pollination with the cryopreserved pollen showed higher fruit and seed set than pollination with pollen stored at 25 °C, and was not significantly different from fresh pollen. This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | cryopreservation Delphinium germination pollen fertility viability |
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