| 人参植株再生体系的建立和优化 |
| |
| 引用本文： | 赵文君,王义,蒋世翠,许媛,孙春玉,张美萍.人参植株再生体系的建立和优化[J].吉林农业大学学报,2009,31(1). |
| |
| 作者姓名： | 赵文君 王义 蒋世翠 许媛 孙春玉 张美萍 |
| |
| 作者单位： | 1. 吉林农业大学生命科学学院,长春,130118;白山经济开发区管理委员会,白山,134300
2. 吉林农业大学生命科学学院,长春,130118 |
| |
| 基金项目： | 吉林省科技发展项目，吉林省中医药管理局资助项目 |
| |
| 摘 要： | 以人参种子、芽胞、茎、叶为外植体,通过器官发生途径建立人参高效再生体系.筛选出不定芽诱导的最佳培养基为MS+2.0 mg/L BA+0.2 mg/L IBA,继代培养基为MS+1.0 mg/L BA+0.2 mg/L IBA和MS+2.5 mg/L BA+0.2 mg/L IBA,生根培养基为1/2 MA+1.0 mg/L NAA;16 h光照/8 h黑暗培养.移栽获得再生植株.
|
| 关 键 词： | 人参 不定芽 植株再生 |
Establishment and Optimization of in vitro Regeneration System for Panax ginseng |
| |
| ZHAO Wen-jun,WANG Yi,JIANG Shi-cui,XU Yuan,SUN Chun-yu,ZHANG Mei-ping.Establishment and Optimization of in vitro Regeneration System for Panax ginseng[J].Journal of Jilin Agricultural University,2009,31(1). |
| |
| Authors: | ZHAO Wen-jun WANG Yi JIANG Shi-cui XU Yuan SUN Chun-yu ZHANG Mei-ping |
| |
| Institution: | 1.College of Life Science;Jilin Agricultural University;Changchun 130118;China;2.Management Committee of Baishan Economic Development Zone;Baishan 134300;China |
| |
| Abstract: | Using seeds,buds,stems and leaves as explants,a regenerative system for shoot morphogensis of Panax ginseng was described.Adventitious bud was induced on MS medium supplemented with MS+2.0 mg/L BA+0.2 mg/L IBA,1.0 mg/L BA and 0.2 mg/L IBA,2.5 mg/L BA and 0.2 mg/L IBA.The root medium is 1/2 MS supplemented with 1.0 mg/L NAA.When adventitious bud was cultured in 16 h illumination and 8 h darkness in turns,regenerative plant was obtained. |
| |
| Keywords: | Panax ginseng adventitious bud regeneration |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
