分离土壤放线菌的方法

用稀释平板法测定土壤中放线菌的数量通常是在淀粉按培养基上进行的,但是采用这种方法在分析某些土壤时,常因细菌和真菌的迅速蔓延,妨碍放线菌发育,以致影响结果的准确性,同时也不易从中得到纯菌。针对这一问题,国内外许多研究工作者曾在修改培养基成分^[1,2],选择抑制剂和运用离心原理^[1]将放线菌和细菌、真菌分离的方法等进行过较多的工作。

A MODIFIED MEDIUM FOR THE ISOLATION OF SOIL ACTINOMYCITES

"Gauze No. 1" medium was used as a culture medium for the isolation of soil actinomycetes with the addition of 100 ppm K₂Cr₂O₇. The modified medium gives larger colony numbers and more kinds of species with distinguishable characteristics in comparison with the original method, but the growth of bacteria and fungi have been largely inhibited. Using this medium, the same species may 'be grouped into species group, and it is possible to identify the names of species group directly on the plate.