室内药剂交替使用对西花蓟马抗药性发展的影响

分别单独使用毒死蜱、多杀菌素和两种农药交替使用连续处理西花蓟马(Frankliniella occidentalis)18代,采用浸渍法从第6至第18代每隔2世代测定3个汰选种群对毒死蜱和多杀菌素的敏感性.结果表明,毒死蜱和多杀菌素交替汰选种群比单一药剂连续汰选种群抗性上升趋势缓慢,F18代时交替使用汰选种群对毒死蜱和多杀菌素的抗性倍数分别为10.89倍和17.19倍,而毒死蜱单一汰选种群对毒死蜱的抗性倍数达到24.19倍,多杀菌素单一汰选种群对多杀菌素的抗性倍数达到20.78倍.因此,交替或轮换使用药剂可以延缓西花蓟马抗药性的发展.

Effects of insecticides with rotational application on resistance development of the western flower thrips,Frankliniella occidentalis

Three different populations of Frankliniella occidentalis were established under rearing conditions through insecticide selection with chlorpyrifos,spinosad and their rotational application for 18 generations,respectively.Toxicity assay from the 6th to the 18th generation of the three different populations to chlorpyrifos and spinosad was performed every two generations by immersion method.The results showed that the resistance developed more slowly when the F.occidentalis was selected with rotational application of chlorpyrifos and spinosad than with single insecticides.The resistance level of the population selected by chlorpyrifos was up to 24.19 fold at the 18th generation,and the population selected by spinosad,20.78 fold.However,the population selected by rotational application of the two pesticides showed 10.89 and 17.19 fold higher resistance to chlorpyrifos and spinosad,respectively.The results suggest that the rational application of insecticides could delay the development of resistance of the western flower thrips to target insecticides.