A novel HPLC method for glutathione-insulin transhydrogenase assay using fluorescence labeled insulin |
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| Authors: | N Saito H Yamanaka S Nagasawa |
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| Affiliation: | National Institute of Animal Health, Ibaraki, Japan. |
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| Abstract: | The activity of rat liver glutathione-insulin transhydrogenase (GIT) was measured by HPLC. The degradation of fluorescein isothiocyanate-I (FITC-I)-labeled insulin is separated into several peaks, which are bound different amount of FITC-I. We selected mono-fluorescein-thiocarbamylated insulin to estimate the decrease of insulin content and it became possible to assay GIT activity. This novel method was time-saving and simple, and this system could utilize instead of previous method. |
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