Over-expression of <Emphasis Type="Italic">Arabidopsis</Emphasis><Emphasis Type="Italic">FT</Emphasis> gene reduces juvenile phase and induces early flowering in ornamental gentian plants |
| |
Authors: | Takashi Nakatsuka Yoshiko Abe Yuko Kakizaki Akiko Kubota Norimoto Shimada Masahiro Nishihara |
| |
Institution: | (1) Iwate Biotechnology Research Center, 22-174-4, Narita, Kitakami Iwate, 024-0003, Japan |
| |
Abstract: | Ornamental gentian plants are perennial and have a juvenile period of over 1 year before flowering. We transformed gentian
plants with a construct comprising the Arabidopsis
FLOWERING LOCUS T (FT) gene (encoding a major component protein of the flowering hormone ‘florigen’) under the control of the rolC promoter from Agrobacterium rhizogenes, which is known to induce vascular-specific expression. The resultant rolCpro-FT transgenic gentian plants showed early flowering in vitro and the earliest line formed floral buds within 4 months
after transformation. Regeneration experiments from leaf explants of these rolCpro-FT transgenic plants also confirmed the early flowering phenotype. After acclimatization, these transgenic plants showed
normal floral development in a closed greenhouse. There is no effective method to induce early flowering by cultivation management
in gentian, therefore these lines might be very useful as annual early season cultivars. |
| |
Keywords: | Early flowering FT Gentian In vitro flowering Transgenic plant |
本文献已被 SpringerLink 等数据库收录! |