趾叶炎小鼠血管内皮生长因子表达的研究

采用组织化学和免疫组化的染色方法,检测趾叶炎趾部组织中血管内皮生长因子表达水平、炎性细胞、肥大细胞及其脱颗粒的动态变化,探讨血管内皮生长因子(VEGF)在趾叶炎发病中的作用。结果表明,二磷酸组织胺注射后炎症组小鼠趾部血管内皮生长因子在真皮网状层、血管及腺体周围和部分肥大细胞内,在14 h表达达到一个峰值,而后表达减弱,72 h~15 d表达迅速增强;炎症组小鼠趾部肥大细胞及其脱颗粒和炎性细胞数量,随二磷酸组织胺注射时间的延长,急剧增多,均极显著高于正常组(P(0.01),且其变化趋势均与血管内皮生长因子表达趋势相吻合;同时在真皮出现了许多增生的小动脉,管壁增厚,管腔内有血栓形成。结果说明血管内皮生长因子参与小鼠趾叶炎的发病过程。

Expression of vascular endothelial growth factor in the mice with digital lobule inflammation

In order to investigate the role of vascular endothelial growth factor(VEGF) in the digital lobule inflammation,the expression level of VEGF and the dynamic changes of inflammatory cells and mast cells and their degranulation in the mice tissues with digital lobule inflammation were detected using the dying methods of histochemistry and immunohistochemistry.The results showed that the expression level of digital VEGF in the mice of inflammatory group reached a peak value in dermal reticular layer,around blood vessels and glands and in some of mast cells at 14 h after injection of histamine diphosphate.Then the expression level of VEGF began to decrease and rapidly increased again from 72 h to 15 d.With the duration of injection,the numbers of mast cells and their degranulation and inflammatory cells at digital parts of the mice in inflammatory group increased rapidly and were significantly higher than those in normal mice(P<0.01).The changes in cell numbers were related to expression of VEGF.At the same time,there were many hyperplastic arteries within hypoderma with thickened vessel wall and thrombosis in cavity.It suggested that VEGF involved in pathogenesis of digital lobule inflammation in mice.