| 柑桔线虫抗性主基因座Tyr1的特异标记开发与遗传作图改进(英文) |
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| 引用本文： | 向旭,邓占鳌,郑启发,陈存贤,Frederick G.Gmitter Jr. 柑桔线虫抗性主基因座Tyr1的特异标记开发与遗传作图改进(英文)[J]. 分子植物育种, 2009, 7(3) |
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| 作者姓名： | 向旭 邓占鳌 郑启发 陈存贤 Frederick G.Gmitter Jr |
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| 作者单位： | [1]广东省农业科学院果树研究所,广州510640 [2]美国佛罗里达大学海岸研究与教育中心,Bradenton,FL34203,美国 [3]美国佛罗里达大学柑桔研究与教育中心,LakeAlfred,FL33850,美国 |
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| 基金项目： | supported in part by grants from the USDA NRICGP (9201765, 9600748);;USDA/National Citrus Research Council (98012205);;the Florida Citrus Production Research Advisory Council (942-27) |
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| 摘 要： | 本文利用先期从BAC文库获得的NBS-LRR类候选抗病基因克隆序列Pt8a和Pt9a，进一步开发与柑桔线虫抗性主效基因位点Tyr1连锁的分子标记。以Pt8a和Pt9a序列作探针，通过高密度克隆印迹杂交，从BAC文库筛选出200个以上的阳性克隆，以阳性克隆插入序列设计引物，对柑桔抗线虫材料和感线虫材料开展以PCR扩增为基础的集群分离分析，发现一部分克隆序列与柑桔线虫抗性主效基因位点Tyr1紧密连锁；再通过染色体步行测序，分别从3个克隆（7A4，4L17和29F20）获得3个完整的NBS-LRR类候选抗病基因序列。从此类序列开发更高特异性的分子标记，并在利用原有分子标记的基础上，对柑桔线虫抗性杂交后代群体（9145 family）构建较高密度的遗传图谱；同时，将新开发的分子标记应用于柑桔衰退病抗性杂交后代群体（9401 family），以初步估算柑桔线虫抗性主效基因位点Tyr]与柑桔衰退病抗性基因Ctv的遗传距离。
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| 关 键 词： | 柑桔 线虫 抗性基因 分子标记 遗传作图 |
Developing Specific Markers and Improving Genetic Mapping for a Major Locus Tyr1 of Citrus Nematode Resistance |
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| Xiang Xu Deng Zhan'ao Zheng Qifa Chen Cunxian Frederick G. Gmitter Jr. Developing Specific Markers and Improving Genetic Mapping for a Major Locus Tyr1 of Citrus Nematode Resistance[J]. Molecular Plant Breeding, 2009, 7(3) |
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| Authors: | Xiang Xu Deng Zhan'ao Zheng Qifa Chen Cunxian Frederick G. Gmitter Jr |
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| Affiliation: | Xiang Xu 1 Deng Zhan'ao 2 Zheng Qifa 3 Chen Cunxian 3 Frederick G. Gmitter Jr. 3 1 Institute of Fruit Tree Research,GDAAS,Guangzhou,510640,2 University of Florida,GCREC,Bradenton,FL34203,USA,3 University of Florida,CREC,Lake Alfred,FL33850 |
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| Abstract: | The NBS-LRR class resistance-gene candidate sequences (Pt8a and Pt9a) were used to develop new specific markers to the citrus nematode resistance gene locus Tyr1. By high-density colony screening, over 200 positive clones were pulled out from the BAC library. A few of the clones were found to be closely linked with the Tyr1 region, because the primers from these clones insert sequence produced polymorphism which matched up with the phenotype after bulked segregant analysis. By primer walking approach, three... |
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| Keywords: | Citrus Nematode Resistance gene Molecular marker Genetic map |
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