| 云南中甸尼西鸡的血液蛋白多态性研究 |
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| 引用本文： | 邹平,张廷钦,宿兵,聂龙,苟世康.云南中甸尼西鸡的血液蛋白多态性研究[J].中国畜牧杂志,1998,34(4):6-9. |
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| 作者姓名： | 邹平 张廷钦 宿兵 聂龙 苟世康 |
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| 作者单位： | 1. 云南省畜牧兽医科学研究所,昆明,650224
2. 中国科学院昆明动物研究所细胞与分子进化研究开放实验室,昆明,650223 |
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| 基金项目： | 云南省应用基础研究重大项目 |
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| 摘 要： | 采用水平式淀粉胶凝胶电泳技术和垂直式聚丙烯酰胺凝胶电泳技术检测了云南中甸尼西鸡的血液蛋白多态，对３３个样本的３７个基因座位进行了分析，共有９个座位出现多态，分别为ＥＳ－１、ＥＳ－２、ＡＫＰ－１、ＡＫＰ－２、ＬＡＰ、ＰＧＭ、ＣＫ、Ｔｆ及６ＰＧＤ。多态位点百分比Ｐ＝０．２４３２，平均杂合度Ｈ＝０．１０１５，每个座位等位基因的平均数Ａ＝１．３７８４，结果表明尼西鸡的血液蛋白多态程度较高，在蛋白质水平上的遗传多样性较为丰富。同时发现Ａｍｙ－１座位只出现杂合子ＡＢ型。
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| 关 键 词： | 中甸尼西鸡 血液蛋白多态 平均杂合度 |
Study on the Blood Protein and lsozyme Polymorphism of the Zhongdian Nixi Chickens in Yunnan Province |
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| Zhou Ping,Zhang Tinqing,Su Bing,Nie Long,Gou Shikang.Study on the Blood Protein and lsozyme Polymorphism of the Zhongdian Nixi Chickens in Yunnan Province[J].Chinese Journal of Animal Science,1998,34(4):6-9. |
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| Authors: | Zhou Ping Zhang Tinqing Su Bing Nie Long Gou Shikang |
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| Abstract: | sing the horizontal starch gel eletrophoresis, Protein and isozyme polymorphism of thirty three blood samples were analyzced of all thc 37 gcnetic loci surveyed 9 of them such as Es-1,Es-2,Akp-1,Akp-2,LAP,PGM,CK,Tf and 6PGD were found to be polymorphic. The other loci were monomorphic. Thc percentagc of polymorphic loci (P) is 0.2432, The mean heterozygosity (H) is 0.1015 and the mean numbcr of allels(A) is 1.3784, The results indicated that the blood protein polymorphism of thc Zhongdian Nixi chickens in Yunnan province is higher. This local chicken breed is wealthy of genetic diversity from the viewpoint of the protein. At Amy l locus, We could find only heterozygous(AB)genotype in thirty three samples. |
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| Keywords: | Zhongdian Nixi Chicken Blood and Protein Isozyme Polymorphism The mean heterozygosity(H) |
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