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| 花生幼胚蛋白质双向电泳的方法与改进 | |
| 引用本文： | 张君诚,蔡晔,张新文,唐荣华,蔡宁波,庄伟建.花生幼胚蛋白质双向电泳的方法与改进[J].山西农业大学学报(自然科学版),2005,25(1):38-41. |
| 作者姓名： | 张君诚 蔡晔 张新文 唐荣华 蔡宁波 庄伟建 |
| 作者单位： | 1. 福建农林大学,作物学院油料研究所,福建,福州,350002;三明高等专科学校,化学与生物工程系,福建,三明,365004 2. 三明高等专科学校,化学与生物工程系,福建,三明,365004 3. 福建农林大学,作物学院油料研究所,福建,福州,350002 |
| 基金项目： | 国家自然科学基金资助项目 (30070481)，福建省教育厅科技项目 (JA02256) |
| 摘 要： | 在对传统的双向电泳方法进行改进和优化的基础上, 探索适合花生早期幼胚蛋白质双向电泳的方法与体系; 利用该方法经考染后能稳定得到100~200个分散性较好的蛋白质斑点, 分子量多集中在20 000~90 000之间。还对一些技术细节进行讨论和分析。 |
| 关 键 词： | 双向电泳 蛋白质 早期幼胚 花生 |
| 文章编号： | 1671-8151(2005)01-0038-04 |
| 修稿时间： | 2004年2月25日 |
An Introduction of the Improvement on the Two-dimensional Electrophoresis for Protein from Early Young Embryo of Peanut |
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| ZHANG Jun-cheng et al..An Introduction of the Improvement on the Two-dimensional Electrophoresis for Protein from Early Young Embryo of Peanut[J].Journal of Shanxi Agricultural University,2005,25(1):38-41. | |
| Authors: | ZHANG Jun-cheng |
| Abstract: | Based on the traditional method of O' Farrell, a improved procedure of two-dimensional gel electrophoresis was introduced.It was proved that the analysis of protein from early young peanut embryo was reliable.With the improved method,100~200 protein spots in good distribution were obtained and molecular weights of the proteins were around the range of 20 000~90 000. The results from it was stable and distinct.Some discussion about the details of procedure was also presented in this paper. |
| Keywords: | Two-dimensional electrophoresis Protein Early young embryo Peanut |
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