|
|||||
|
|
| Identification of Self-Incompatibility Genotypes in Some Sand Pears （Pyrus pyrifolia Nakai） by PCR-RFLP Analysis | |
| 引用本文： | GU Qing-qing,ZHANG Qing-lin,HU Hong-jü,CHEN Qi-liang,LUO Zheng-rong.Identification of Self-Incompatibility Genotypes in Some Sand Pears （Pyrus pyrifolia Nakai） by PCR-RFLP Analysis[J].中国农业科学(英文版),2009,8(2):154-160. |
| 作者姓名： | GU Qing-qing ZHANG Qing-lin HU Hong-jü CHEN Qi-liang LUO Zheng-rong |
| 作者单位： | GU Qing-qing(Key Laboratory for Horticultural Plant Biology of Ministry of Education (MOE)/Huazhong Agricultural University, Wuhan 430070, P.R. China;Department of Horticulture, Jiangxi Agricultural University, Nanchang 330045, P.R.China);ZHANG Qing-lin,LUO Zheng-rong(Key Laboratory for Horticultural Plant Biology of Ministry of Education (MOE)/Huazhong Agricultural University, Wuhan 430070, P.R. China);HU Hong-ju,CHEN Qi-liang(Fruit-Tree and Tea Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430209, P.R.China) |
| 基金项目： | 江西农业大学自然科学基金 |
| 摘 要： | The identification of self-incompatibility genotype （S-genotype） will be useful for selection of pollinizers and design of crossing in cultivar improvement of sand pear. This paper reported the identification of self-incompatibility genotypes of seven Chinese and two Japanese sand pear cultivars using PCR-RFLP analysis and S-RNase sequencing. The Sgenotypes of these cultivars were determined as follows： Huali 1 S1S3, Shounan S1S3, Xizilti S1S4, Qingxiang S3S7, Sanhua S2S7, Huangmi （Imamuranatsu） S1S6, Huali 2 S3S4, Baozhuli S7S33, Cangxixueli S5S15. S-RNase alleles （S1 to S9） in sand pear could be identified effectively by PCR-RFLP analysis. |
| 关 键 词： | 自交不亲和性 RFLP分析 砂梨品种 PCR 基因型 核糖核酸酶 识别 核酸酶基因 |
| 收稿时间： | 26 March 2008 |
Identification of Self-Incompatibility Genotypes in Some Sand Pears (Pyrus pyrifolia Nakai) by PCR-RFLP Analysis |
|
| GU Qing-qing,ZHANG Qing-lin,HU Hong-ju,CHEN Qi-liang,LUO Zheng-rong.Identification of Self-Incompatibility Genotypes in Some Sand Pears (Pyrus pyrifolia Nakai) by PCR-RFLP Analysis[J].Agricultural Sciences in China,2009,8(2):154-160. | |
| Authors: | GU Qing-qing ZHANG Qing-lin HU Hong-ju CHEN Qi-liang LUO Zheng-rong |
| Institution: | 1. Key Laboratory for Horticultural Plant Biology of Ministry of Education (MOE)/Huazhong Agricultural University, Wuhan 430070, P.R. China;Department of Horticulture, Jiangxi Agricultural University, Nanchang 330045, P.R.China 2. Key Laboratory for Horticultural Plant Biology of Ministry of Education (MOE)/Huazhong Agricultural University, Wuhan 430070, P.R. China 3. Fruit-Tree and Tea Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430209, P.R.China |
| Abstract: | The identification of self-incompatibility genotype (S-genotype) will be useful for selection of pollinizers and design of crossing in cultivar improvement of sand pear. This paper reported the identification of self-incompatibility genotypes of seven Chinese and two Japanese sand pear cultivars using PCR-RFLP analysis and S-Rnase sequencing. The S-genotypes of these cultivars were determined as follows: Huali 1 S1S3, Shounan S1S3, Xizili S1S4, Qingxiang S3S7, Sanhua S2S7, Huangmi (Imamuranatsu) S1S6, Huali 2 S3S4, Baozhuli S7S33, Cangxixueli S5S15. S-Rnase alleles (S1 to S9) in sand pear could be identified effectively by PCR-RFLP analysis. |
| Keywords: | Pyrus pyrifolia Nakai self-incompatibility genotype S-allele S-Rnase PCR-RFLP crossing experiment |
| 本文献已被 维普 万方数据 ScienceDirect 等数据库收录！ | |