| 湖北柑橘溃疡病菌的PCR鉴定及序列分析 |
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| 引用本文： | 罗志萍,洪霓. 湖北柑橘溃疡病菌的PCR鉴定及序列分析[J]. 湖北农业科学, 2006, 45(5): 542-545 |
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| 作者姓名： | 罗志萍 洪霓 |
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| 作者单位： | 湖北出入境检验检疫局,武汉,430022;华中农业大学柑橘脱毒中心,武汉,430070 |
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| 基金项目： | 江苏省中医药管理局资助项目 |
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| 摘 要： | 选用引物JYF5/JYR5对湖北疑似柑橘溃疡病标本进行了PCR鉴定和确证,同时改进了DNA抽提方法,优化了PCR条件,并与南京柑橘溃疡病菌株进行分子克隆比较.结果表明,南京菌株NJ-XC分离物扩增片段与Silva等报道的相应序列相似性为99%(411/413);湖北标本菌株HB-X0扩增片段与报道的相应序列相似性为100%(413/413),二者存在一定的差异.此外发现引物JYF5/JYR5能扩增叶表的根癌土壤杆菌,其片段大小为575bp.
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| 关 键 词： | 柑橘溃疡病菌 PCR鉴定 序列分析 |
| 文章编号： | 0439-8114(2006)05-0542-04 |
| 收稿时间： | 2006-06-28 |
| 修稿时间： | 2006-06-28 |
Identification and Sequence Andysis of Hubei CBCD(Xanthomonas axonopodis pv citrus)by PCR |
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| LUO Zhi-ping,HONG Ni. Identification and Sequence Andysis of Hubei CBCD(Xanthomonas axonopodis pv citrus)by PCR[J]. Hubei Agricultural Sciences, 2006, 45(5): 542-545 |
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| Authors: | LUO Zhi-ping HONG Ni |
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| Affiliation: | 1.Hubei Enfry-Ezit Inspection and Quarantine,Wuhan 430022,China; 2.Research Center of Virus-free Orange,Huazhong Agricultural University,Wuhan 430070,China |
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| Abstract: | In this paper,a citrus sample from Hubei was identified by PCR Assay.At the same time,the method of DNA extraction was developed,and it was cloned and sequenced to make a comparison with Xac from Nanjing.The result showed that the two different strains had certain discrepancy in sequences.The comparability between the Nanjing sequence and that reported by Silva(2002)was 99 percent(411/413);the comparability between the Hubei sequence and the reported was 100 percent(413/413).Under the same primer,the amplified fragments of and unknown stain derived from the disease spots isolation showed high comparability of 93 percent(484/515)with one section of Agrobacterium tumefaciens sequences(575bp). |
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| Keywords: | Xanthomonas axonopodis pv citrus PCR sequence |
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