RAPD Patterns Characteristic for VCGs of Cotton Verticillium Wilt Pathogen, Verticillium dahliae |
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Authors: | WANG Ke-rong CHEN Rui-hui |
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Institution: | 1. Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, P. R . China 2. Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, P. R . China;Taichou Entry-Exit Inspection and Quarantine Bureau, Taizhou 225300, P. R . China |
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Abstract: | Nine primers were employed to detect molecular polymorphisms in 103 Verticillium dahliae isolates that represent diverse groups of Vegetative Compatibility (VC). Our results showed that these isolates confer two distinctive RAPD groups (RPGs). RPG1, consists of isolates belonging to vegetative compatibility group Ⅰ (VCG Ⅰ), while RPG2 encompasses VCGⅡ and VCGⅣ. The genetic diversity associated with VCGⅢ was greater than that associated with VCG1. Five RAPD fragments, with frequencies more than 0.96 in VCG Ⅰ but less than 0.1 in VCGⅢ, produced characteristic fragments for VCG Ⅰ (defoliating type), suggesting strong correlation between RPGs and VCGs. |
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Keywords: | Verticillium dahliae Vegetative compatibility Defoliating type RAPD |
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