舌鰕虎鱼的人工繁殖及其胚胎发育

为掌握舌鰕虎鱼Glossogobius giuris的人工繁殖技术及其胚胎发育特点,进行了舌鰕虎鱼人工繁殖试验和胚胎发育观察,研究了舌鰕虎鱼亲鱼强化培育、人工催产、产卵受精和胚胎发育过程,并描述了胚胎各发育时期的时序和形态特征。结果表明:性腺成熟的舌鰕虎鱼亲鱼经两次激素注射催熟催产,催产率为60%~80%,受精率为73.4%~81.2%,孵化率为63.8%~77.2%;舌鰕虎鱼受精卵为纺锤形,呈黏性且半透明;胚胎发育分为胚盘形成期、卵裂期、囊胚期、原肠期、神经胚期、器官形成期和出膜期7个阶段,以及26个发育时期;在25~27℃条件下需要108 h完成孵化过程,初孵仔鱼体长为2.5~3.5 mm,全长为2.7~3.8 mm,肌节为26~28对,心跳为50~55次/min。本研究结果可为舌鰕虎鱼资源的保护和人工繁殖提供理论指导。

Artificial propagation and embryonic development of forktonge goby Glossogobius giuris

The brood stock rearing, artificial spawning, fertilization and embryonic development of forktonge goby Glossogobius giuris were studied to develop artificial breeding techniques of forktonge goby. The development time and morphological characteristics of each embryonic development stage were described. The ripe forktonge goby were induced to spawn by twice injections of hormone, with spawning success of 60%-80%, fertilization rate of 73. 4%-81. 2% and hatching rate of 63. 8%-77. 2%. The fertilized eggs were translucent, adhensive and fusi-form in shape, and the embryonic development was divided into 7 physiological stages includding fertilized egg stage,cleavage stage, blastula stage, gastrula stage, neural stage, organogenesis stage and hatching stage with 26 substages. The fertilized eggs were hatched in 108 hours at 25-27 ℃. Newly hatehed larvae had average body length of 2. 5-3. 5 mm,total length of 2. 7-3. 8 mm with about 26-28 myomeres and heart beating rate of 50-55 times per minute. The findings might provide theoretical guidance for protection of resources and artificial breeding of forktonge goby.