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| 山东百部离体快繁技术的研究 | |
| 引用本文： | 张建宇,张鹏,段祖安,王海朋,羿德磊,赵艳燕,潘文兵.山东百部离体快繁技术的研究[J].山东林业科技,2010,40(2):45-47. |
| 作者姓名： | 张建宇 张鹏 段祖安 王海朋 羿德磊 赵艳燕 潘文兵 |
| 作者单位： | 1. 临沂市园林管理局,276000 2. 山东省林业监测规划院 3. 山东农业大学林学院 |
| 摘 要： | 以山东百部(Stemona Shandongensis D.K.Zang)茎段为外植体,进行离体快繁技术的研究。实验结果表明:启动培养基为MS+6-BA 5.0mg.L-1+NAA0.1mg.L-1+糖30g.L-1+琼脂6.5g.L-1;分化培养基为MS+6-BA 4.0mg.L-1+NAA0.05mg.L-1+糖30g.L-1+琼脂6.5g.L-1;生根培养基为1/2MS+NAA0.1mg.L-1+糖30g.L-1+琼脂6.5g.L-1,通过正交试验也证明了实验结果。 |
| 关 键 词： | 山东百部 组织培养 正交试验 |
Study on the Art of Stem in Vitro Tissue Culture |
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| Zhang Jianyu,Zhang Peng,Duan Zuan,Wang Haipeng,Yi Delei,Zhao Yanyan,Pan Wenbing.Study on the Art of Stem in Vitro Tissue Culture[J].Journal of Shandong Forestry Science and Technology,2010,40(2):45-47. | |
| Authors: | Zhang Jianyu Zhang Peng Duan Zuan Wang Haipeng Yi Delei Zhao Yanyan Pan Wenbing |
| Institution: | Zhang Jianyu1,Zhang Peng2,Duan Zuan3,Wang Haipeng3,Yi Delei3,Zhao Yanyan3,Pan Wenbing2(1.Linyi City Park Authority,2.Forestry Bureau of Sh,ong Province,3.College of Forestry,Sh,ong Agricultural University) |
| Abstract: | Using the stem（Stemona Shandongensis D. K. Zang）buds as explants, this paper study on the art of stem in vitro tissue culture. The results shows that the appropriate initial medium is MS＋6--BA 5.0mg . L -1 ＋NAA 0. 1mg . L-1 ＋ sucrose30g. L-1＋agar 6.5g. L-1 ;The medium of polarize is MS＋6-BA4.0mg. L-1＋NAA0.05mg. L-1 ＋sucrose 30g. L-1＋agar 6.5g. L -1;The medium producing root is 1/2MS＋NAA 0.1mg.L-1＋sucrose 30g . L -1＋agar 6.5g . L-1. The tested by orthogonal also proved the results . |
| Keywords: | Stemona Shandongensis D K Zang Tissue Culture Orthogonal Tests |
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