|
|||||
|
|
| 高效液相色谱法测定防霉剂中丙酸含量的研究 | |
| 引用本文： | 姜涛,潘红红,李辉章,刘云花,盛强,胡建蓉,王鑫.高效液相色谱法测定防霉剂中丙酸含量的研究[J].粮食储藏,2011,40(5):32-34. |
| 作者姓名： | 姜涛 潘红红 李辉章 刘云花 盛强 胡建蓉 王鑫 |
| 作者单位： | 1. 中储粮成都粮食储藏科学研究所,四川成都,610031 2. 成都理工大学,四川成都,610059 3. 成都市产品质量监督检验院,四川成都,610041 |
| 摘 要： | 研究建立了使用高效液相色谱法测定防霉剂中丙酸含量的方法。样品用水超声提取,经C18柱（150mm×4.6mm 5um）分离,通过紫外-可见检测器在210nm处定量测定。结果表明,在0.5mg/mL～5.0mg/mL浓度范围内,丙酸浓度与峰面积的回归方程为：y=329.40x-12.281,相关系数r=0.9994,回收率98.6%～101.7%。对丙酸含量不同的防霉剂,与使用GB/T22145-2008（气相色谱法）相比,方法间的检测结果相对偏差为0.41%～1.13%。实验证明：该方法简单、快速、准确性好,精密度高,可用于测定防霉剂中丙酸含量。 |
| 关 键 词： | 防霉剂 丙酸 液相色谱法 |
DETERMINATION OF PROPIONIC ACID CONTENT IN MOULD INHIBITOR BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY(HPLC) |
|
| Jiang Tao,Pan Honghong,Li Huizhang,Liu Yunhua,Sheng Qiang,Hu Jianrong,Wang Xin.DETERMINATION OF PROPIONIC ACID CONTENT IN MOULD INHIBITOR BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY(HPLC)[J].Grain Storage,2011,40(5):32-34. | |
| Authors: | Jiang Tao Pan Honghong Li Huizhang Liu Yunhua Sheng Qiang Hu Jianrong Wang Xin |
| Institution: | Jiang Tao1 Pan Honghong2 Li Huizhang1 Liu Yunhua1 Sheng Qiang1 Hu Jianrong1 Wang Xin3(1 Chengdu Grain Storage Research Institute,China Grain Reserves Co.610031)(2 Chengdu University of Technology 610059)(3 Chengdu Product Quality Supervision and Inspection Institute 610041) |
| Abstract: | The determination method of propionic acid content in mould inhibitor by HPLC was established.Samples were extracted by ultrasonic method,separated by column C18（150mm×4.6mm×5μm）,made quantitative measurement at 210nm by UV-Vis detector.The results showed that in the concentration range from 0.5 mg/mL to 5.0mg/mL,the regression equation of propionic acid concentration and the peak area was obtained as y=329.40x-12.281,which the correlative index（r） was 0.9994,the average recovery was from 98.6% to 101.7%.For mould inhibitor with different propionic acid content,comparing with GB/T22145-2008（Gas Chromatography）,the test results showed that the relative deviation was from 0.41% to 1.13% between the different methods.The method was proved to be easy,fast,good accuracy and high precision,it could be used to determinate the propionic acid content in mould inhibitor. |
| Keywords: | mould inhibitor propionic acid High-performance liquid chromatography |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |