| 兔病毒性出血症鸡源高免蛋黄抗体的研究与应用 |
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| 引用本文： | 王孝友,徐刚,何洪章,孔路军,曾秀,黄伟. 兔病毒性出血症鸡源高免蛋黄抗体的研究与应用[J]. 中国养兔杂志, 2004, 0(4): 20-22 |
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| 作者姓名： | 王孝友 徐刚 何洪章 孔路军 曾秀 黄伟 |
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| 作者单位： | [1]重庆市养猪科学研究院，重庆402400 [2]西南农业大学荣昌校区，重庆402460 [3]重庆市永健生物技术有限责任公司，重庆402400 |
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| 摘 要： | 用兔病毒性出血症抗原免疫产蛋鸡,收集高免蛋,精制蛋黄抗体(IgY),采用血凝抑制试验(HI)测定IgY的最高滴度为15 1og2.通过IgY的被动免疫保护试验证实,IgY滴度稀释至13 1og2时肌肉注射体重2.0～2.5kg的健康非免家兔5.0mL/只,可抵抗兔病毒性出血症强毒RC株1.0mL(血凝价HA为9 1og2)的攻击.临床应用效果显示:IgY(HI为13 log2)对发病兔进行紧急防治,治愈率为84.38%～92.86%,能迅速控制疫情.
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| 关 键 词： | 兔病毒性出血症 蛋黄抗体 |
| 修稿时间： | 2004-05-17 |
Research and Appalication of Rabbit Haemorrhagic Disease Virus High -lever Immunized Yolk Antibody |
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| High- lever Immunized Yolk AntibodyWang Xiaoyou Xu Gang He Hongzhang Kong Lujun Zeng Xiu Huang Wei. Research and Appalication of Rabbit Haemorrhagic Disease Virus High -lever Immunized Yolk Antibody[J]. Chinese Journal of Rabbit Farming(CJRF), 2004, 0(4): 20-22 |
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| Authors: | High- lever Immunized Yolk AntibodyWang Xiaoyou Xu Gang He Hongzhang Kong Lujun Zeng Xiu Huang Wei |
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| Affiliation: | High- lever Immunized Yolk AntibodyWang Xiaoyou1 Xu Gang3 He Hongzhang2 Kong Lujun1 Zeng Xiu1 Huang Wei3 |
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| Abstract: | A flow full- grown hens were immunized with inactivated rabbit haemorrhagic disease virus(RHDV).After a boosting process eggs were collected and used to extract the special yolk antibody(IgY).The maximum titer of this preparation reached 15 1og2 by hemagglutination inhibition assay(HI).It was confirmed through passive immunization of IgY that healthy nonimmunized rabbits whose weight varied during 2.0kg and 2.5kg were intramuscularinjected with IgY 5.0mL(HI=13 1og2),they could resist the attact of 1.0 mL(HA= 9 1og2)RHDV- RC.The clinical application effect of IgY(HI=13 1og2)showed the cure rate was 84.38 to 92.86 percent to diseased rabbits and they could control the epidemic situation immediately. |
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| Keywords: | Rabbit haemorrhagic disease Yolk antibody |
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