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| 柑橘裂皮病类病毒的一步RT-PCR法检测及其在甜橙体内的分布 | |
| 引用本文： | 彭军,周常勇,唐科志,谭万忠. 柑橘裂皮病类病毒的一步RT-PCR法检测及其在甜橙体内的分布[J]. 果树学报, 2005, 22(6): 744-747 |
| 作者姓名： | 彭军 周常勇 唐科志 谭万忠 |
| 作者单位： | 1. 中国农业科学院柑橘研究所,重庆,400712;西南农业大学植物保护学院,重庆,400716 2. 中国农业科学院柑橘研究所,重庆,400712 3. 西南农业大学植物保护学院,重庆,400716 |
| 摘 要： | 柑橘裂皮病是由柑橘裂皮病类病毒(Citrusexocortisviroid,CEVd)引起的一种重要的柑橘病害。分别采用快速微量核酸提取法和SDS-KAc抽提法在表现症状的Etrog香橼中提取核酸,采用一步RT-PCR法对CEVd进行检测,结果显示SDS-KAc法可以消除带毒样品中非特异性条带。从嫁接接毒的铜水72-1锦橙植株的接穗部取嫩叶、老叶、皮,从砧木部茎杆上取老皮以及根皮分别提取总核酸进行RT-PCR检测,分析CEVd在甜橙体内的分布情况。初步检测结果表明在接穗的嫩叶、老叶、皮,砧木部茎杆的老皮和根皮上都可以检测到CEVd,以接穗部叶和皮检出稳定性高。 |
| 关 键 词： | 柑橘裂皮病类病毒 分布 甜橙 RT-PCR检测 |
| 文章编号： | 1009-9980(2005)06-744-04 |
| 修稿时间： | 2004-04-20 |
Preliminary study on One-step RT-PCR detection of Citrus exocortis viroid (CEVd) and its distribution in different parts of sweet orange |
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| PENG Jun,ZHOU Chang-yong,TANG Ke-zhi,TAN Wan-zhong. Preliminary study on One-step RT-PCR detection of Citrus exocortis viroid (CEVd) and its distribution in different parts of sweet orange[J]. Journal of Fruit Science, 2005, 22(6): 744-747 | |
| Authors: | PENG Jun ZHOU Chang-yong TANG Ke-zhi TAN Wan-zhong |
| Affiliation: | PENG Jun1,2,ZHOU Chang-yong1,2*,TANG Ke-zhi1,TAN Wan-zhong2 |
| Abstract: | Citrus exocortis is an important viroid disease. Preliminary study on comparison of Fast Micro-extraction method and SDS-KAc method,detecting CEVd in Etrog citron with symptoms by One-step RT-PCR, revealed that SDS-KAc method was preferable for eliminating non-specific band. Distribution of CEVd in the young leaf, mature leaf, bark and rootlet was monitored by One-step RT-PCR. The results indicated that CEVd could be detected in all parts monitored by One-step PT-PCR. Considering stability, leaf and bark of scion are more suitable to be used for detection of CEVd by One-step RT-PCR. |
| Keywords: | Citrus exocortis viroid Distribution Sweet orange RT-PCR detection |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |