A spruce defensin showing strong antifungal activity and increased transcript accumulation after wounding and jasmonate treatments |
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| Authors: | Isabelle Pervieux, Martin Bourassa, Fran oise Laurans, Richard Hamelin,Armand S guin |
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| Affiliation: | aNatural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, P.O. Box 3800, 1055 du PEPS, Sainte-Foy, Que., Canada G1V 4C7;bINRA Orléans, Unité Amélioration, Génétique et Physiologie Forestières, Avenue de la Pomme de Pin, BP 20619 Ardon, 45166 Olivet Cedex, France |
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| Abstract: | In this work, a cDNA encoding a defensin from Picea glauca, named PgD1, was isolated. The corresponding PgD1 cDNA encodes a putative protein of 83 amino acids with a calculated molecular mass of 8.9 kDa. The mature protein of 50 amino acids is characterized by the presence of specific highly conserved residues common to all plant defensins. PgD1 is developmentally expressed during seed germination and is also up-regulated by wounding and by jasmonic acid treatment, suggesting a role in both the constitutive and induced defense mechanisms. The recombinant protein was found to cause extensive growth inhibition of three fungal pathogens (Cylindrocladium floridanum, Fusarium oxysporum and Nectria galligena) at 2.5 μM. |
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| Keywords: | Jasmonic acid Methyl jasmonate Picea Defensin Wounding |
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