氟苯尼考胺人工抗原的合成与鉴定

[目的]合成并鉴定氟苯尼考胺人工抗原.[方法]采用水解的方法制备了氟苯尼考胺,并通过紫外、红外、质谱鉴定氟苯尼考胺水解成功.采用戊二醛法合成了氟苯尼考人工抗原,并通过紫外光谱进行鉴定.免疫大白兔制备了氟苯尼考胺高特异性抗体.[结果]通过紫外、红外、质谱鉴定氟苯尼考胺水解成功,抗体效价达6.4×104.[结论]氟苯尼考胺人工抗原合成成功,为建立FFA免疫化学方法奠定了技术基础.

Synthesis and Identification of Artificial Antigen of Florfenicol amine

[Objective] The research aimed to prepare and identify the artificial antigen of florfenicol amine.[Method] The artificial antigen of florfenicol amine was synthesized by using hydrolysis method.The product was examined by ultraviolet spectrum,infrared spectrum and mass.FFA was covalently attached to carrier protein as immunogen by glutaraldehyde method.The conjugates was identified with ultraviolet spectrum.FFA-BSA was used to immune New Zealand white rabbits and prepare polyclonal antibody of FFA with high affinity and high specificity.[Result] The results showed these products were synthesized successfully.The antibodies produced with FFA-BSA conjugate showed titer of 6.4 × 104.[Conclusion] The synthesis route of FFA artificial antigen was feasible.This research could lay the foundation for further establishment of the immune detection method for FFA.