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盐胁迫对小麦过氧化物酶同工酶基因表达的影响
引用本文:孙静,王宪泽.盐胁迫对小麦过氧化物酶同工酶基因表达的影响[J].麦类作物学报,2006,26(1):42-44,61.
作者姓名:孙静  王宪泽
作者单位:1. 山东农业大学,山东泰安,271018;莱芜职业技术学院,山东莱芜,271100
2. 山东农业大学,山东泰安,271018
基金项目:山东省"三О"工程项目
摘    要:为了研究小麦过氧化物酶同工酶基因表达与盐适应性的关系,以小麦品种pH-82—2—2为材料对盐胁迫下过氧化物同工酶谱的变化进行了分析,结果表明,PH-82—2—2经盐胁迫后,根、叶中过氧化物酶同工酶发生显著变化,说明盐胁迫影响该基因的表达。盐胁迫对5个同工酶14个等位基因的表达产生不同的影响.有些基因只有在盐胁迫下才表达,如Px-2b等,有些基因的表达具有组织器官特异性。如Px-1等。盐胁迫下过氧化物酶同工酶基因表达水平的变化,说明小麦遭受盐胁迫后会通过控制基因的表达达到抵抗盐渍侵害的作用。

关 键 词:小麦  盐胁迫  过氧化物酶同工酶  基因表达
文章编号:1009-1041(2006)01-0042-04
收稿时间:2005-04-20
修稿时间:2005-04-202005-09-01

Effects of Salt Stress on Gene Expression of Peroxidase Isozyme in Wheat
SUN Jing,WANG Xian-ze.Effects of Salt Stress on Gene Expression of Peroxidase Isozyme in Wheat[J].Journal of Triticeae Crops,2006,26(1):42-44,61.
Authors:SUN Jing  WANG Xian-ze
Institution:1. Shandong Agricultural University, Taian, Shandong 271000, China; 2. Laiwu Vocational Technology College, Laiwu, Shandong 271100, China
Abstract:Using the method of polyacrylamide gel electrophoresis(PAGE),POD isozeymes in different tissue were analyzed in PH-82-2-2 wheat cultivar under salt stress.Great changes were found in POD isoenzyme,which indicated that there were close relationships between salt-stress and gene expression.A total of 14 alleles at 5 loci were influenced on expression under saltstress.P_X-2b expressed only under salt stress,and P_X-1 expressed only in specific tissue.All the reaction indicated that the salt resistance of wheat cultivars were raised by some gene expression.
Keywords:Wheat  Salt-stress  Peroxidase isozyme  Gene expression
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