Blue-native PAGE in plants: a tool in analysis of protein-protein interactions |
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Authors: | Eubel Holger Braun Hans-Peter Millar A Harvey |
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Institution: | ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, 35 Stirling Hwy, Crawley 6009, Perth, Australia. |
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Abstract: | Intact protein complexes can be separated by apparent molecular mass using a standard polyacrylamide gel electrophoresis system
combining mild detergents and the dye Coomassie Blue. Referring to the blue coloured gel and the gentle method of solubilization
yielding native and enzymatically active protein complexes, this technique has been named Blue-Native Polyacrylamide Gel-Electrophoresis
(BN-PAGE). BN-PAGE has become the method of choice for the investigation of the respiratory protein complexes of the electron
transfer chains of a range of organisms, including bacteria, yeasts, animals and plants. It allows the separation in two dimensions
of extremely hydrophobic protein sets for analysis and also provides information on their native interactions. In this review
we discuss the capabilities of BN-PAGE in proteomics and the wider investigation of protein:protein interactions with a focus
on its use and potential in plant science. |
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