提高籼稻花培绿苗诱导率的研究 Ⅰ.激素处理籼稻植株对花粉绿苗诱导率的影响

用赤霉酸(GA_3),乙烯利(CPA)、三十烷醇(TRIA)、脱落酸(ABA)、2,4-D 以及6-苄基嘌呤(BA)等激素处理孕穗植株,共处理14份籼稻材料。其中 GA_3和 CPA 对11份材科作了重复试验,证明 GA_3和 CPA 对大部分材料能大幅度提高花培绿苗诱导率,其最适浓度分别为4～7 ppm 和400ppm,但 GA_3、CPA 以及低温预处理,对少数材料的花培诱导有抑制作用。低浓度 TRIA 与 GA_3混合液的处理效果明显优于单独用 GA_3处理的。其它激素除 ABA 有一定作用外,未取得良好效果.

STUDIES ON INCREASING THE RATE OF ANTHER CULTURE TO GREEN PLANTLETS OF HSIEN RICE Ⅰ.An Investigation on the Hsien Rice Seedling with Growth Hormones to Increase the Induction Rate of Anther Culture

Young panicles of Hsien rice at the meiotic stage were spraying with gibberellic acid(GA_3),ethrel(CPA),triacontanol(TRIA)and abscisic acid(ABA),2,4-D,6-benzyladenine(6-BA).Total 14 materials of Hsien rice were treated,11 materials were repeated test by GA_3 and CPA to effect of the rate of pollen green plantlets.The results showed that, with GA_3 and CPA spraying the many materials promoted the induction rate to pollen green plantlets in a great range.Testify 11 materials of Hsien by GA_3 4-7 ppm and CPA 400 ppm got a better effect than low temper- ature pretreatment.The few materials were inhibited by GA_3 and CPA.But treated with low concentration of TRIA(0.3 ppm)mixed with GA_3(4 ppm) showed significantly were efficiency of green buds differentitated better thansimple GA_3.The effect of the other hormones(except ABA)were non- significance.