| SSR标记技术在玉米杂交种种子纯度测定中的应用 |
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| 引用本文： | 李晓辉,李新海,李文华,王振华,马凤鸣,袁力行,张世煌.SSR标记技术在玉米杂交种种子纯度测定中的应用[J].作物学报,2003,29(1):63-68. |
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| 作者姓名： | 李晓辉 李新海 李文华 王振华 马凤鸣 袁力行 张世煌 |
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| 作者单位： | 摘要: 以21份玉米骨干自交系及其组配的13个杂交种为材料, 进行SSR标记分析. 从220对引物中, 筛选出扩增带型稳定、多态性丰富的58对引物; 杂交种的SSR图谱基本上表现为双亲互补带型. 利用两个或三个引物组合构建的SSR图谱, 通过统计测验可以将13个杂交种区分. 实验表明, 应用SSR标记技术结合单籽粒DNA快速提取方法, 可以快捷、准确 |
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| 基金项目： | 科技部重点项目 (K2 0 0 0 -2 0 -4 3 )，亚洲玉米生物技术协作网项目 (AMBIONET)资助 |
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| 摘 要： | 以21份玉米骨干自交系及其组配的13个杂交种为材料, 进行SSR标记分析. 从220对引物中, 筛选出扩增带型稳定、多态性丰富的58对引物; 杂交种的SSR图谱基本上表现为双亲互补带型. 利用两个或三个引物组合构建的SSR图谱, 通过统计测验可以将13个杂交种区分. 实验表明, 应用SSR标记技术结合单籽粒DNA快速提取方法, 可以快捷、准确
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| 关 键 词： | 玉米 SSR标记 杂交种纯度鉴定 |
| 收稿时间： | 2001-11-23 |
| 修稿时间： | 2001年11月23 |
Application of SSR Markers in Hybrid Seed Purity Test of Maize |
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| LI Xiao Hui,LI Xin Hai,LI Wen Hua,WANG Zhen Hua,MA Feng Ming,YUAN Li Xing,ZHANG Shi Huang.Application of SSR Markers in Hybrid Seed Purity Test of Maize[J].Acta Agronomica Sinica,2003,29(1):63-68. |
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| Authors: | LI Xiao Hui LI Xin Hai LI Wen Hua WANG Zhen Hua MA Feng Ming YUAN Li Xing ZHANG Shi Huang |
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| Institution: | LI Xiao Hui 2 LI Xin Hai 1 LI Wen Hua 2 WANG Zhen Hua 2 MA Feng Ming 2 YUAN Li Xing 1 ZHANG Shi Huang 1 |
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| Abstract: | The polymorphism of 21 maize elite inbred lines and 13 hybrids were analyzed with SSR marker system. Fifty eight out of 220 pairs of SSR primers were selected, which gave stable and polymorphic amplification profiles. Basically, the amplification bands of hybrids were complementary with that of the parents. Thirteen hybrids could be distinguished by combinations of two or three primers selected and statistical analysis. The rapid DNA extracting procedure from single kernel was established. The experiment showed that SSR technique could be effectively exploited in hybrid seed purity test of maize. |
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| Keywords: | Zea may L Simple sequence repeats maker Hybrid seed purity test |
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