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| 口蹄疫疫苗效检模型动物测毒及口蹄疫种毒冻干试验 | |
| 引用本文： | 郎洪武,支海兵,闫岩,苏志堂,李平,陈兴芳,杨汉春.口蹄疫疫苗效检模型动物测毒及口蹄疫种毒冻干试验[J].中国兽药杂志,2004,38(5):24-26. |
| 作者姓名： | 郎洪武 支海兵 闫岩 苏志堂 李平 陈兴芳 杨汉春 |
| 作者单位： | 1. 中国兽医药品监察所,北京,100081 2. 中牧股份兰州生物药厂,甘肃,兰州,730046 3. 中国农业大学,北京,100094 |
| 基金项目： | 农业部畜牧兽医局资助项目 |
| 摘 要： | 应用豚鼠作为口蹄疫疫苗效检模型动物检测口蹄疫病毒对模型动物的病原性.用体重400 g左右的豚鼠,将猪O型口蹄疫灭活疫苗效检攻毒毒株ORMF8经后肢蹠部皮内注射途径进行测毒.测毒结果表明,口蹄疫病毒可引起豚鼠出现典型发病,并产生明显病变,ORMF8种毒对豚鼠的毒价可达105.5ID50/0.2 mL.将乳鼠中和试验用种毒OMⅡ按一定比例加入5%蔗糖脱脂牛奶稳定剂进行冷冻真空干燥试验,3次冻干试验结果表明,种毒冻干后病毒含量有一定程度的下降,但下降程度不显著. |
| 关 键 词： | 口蹄疫疫苗 模型动物 测毒 冻干试验 |
| 文章编号： | 1002-1280(2004)05-0024-03 |
| 修稿时间： | 2003年7月8日 |
Virulence detection of challenge virus on guinea pigs used as model animals for potency tests of FMD vaccine and the freeze-drying tests of FMD virus |
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| LANG Hong-wu,ZHI Hai-bing,YAN yan,SU Zhi-tang,LI Ping,CHEN Xing-fang,YANG Han-chun.Virulence detection of challenge virus on guinea pigs used as model animals for potency tests of FMD vaccine and the freeze-drying tests of FMD virus[J].Chinese Journal of Veterinary Drug,2004,38(5):24-26. | |
| Authors: | LANG Hong-wu ZHI Hai-bing YAN yan SU Zhi-tang LI Ping CHEN Xing-fang YANG Han-chun |
| Institution: | LANG Hong-wu~1,ZHI Hai-bing~1,YAN yan~2,SU Zhi-tang~2,LI Ping~2,CHEN Xing-fang~2,YANG Han-chun~3 |
| Abstract: | The guinea pigs assumed as model animals used on the potency tests of foot and mouth disease(FMD) vaccine were detected on their pathogenicity with FMD virus.The guinea pigs avoirdupois about 400 g were (challenged) by intradermal injection into the heel bulbs with strain FMD ORMF_8.The results showed that FMD (virus) strain ORMF_8 can make the guinea pigs emergence representative FMD infectious disease symptoms and pathological changes. The titer of ORMF_8 can reach 10~(5.5) ID_(50)/0.2 mL. Add stable reagent of degrease milk (according) to a certainty proportion to the seed of OMII FMD and then test the freeze-drying vaccine three times. The results showed that the titer of OMII FMD had a certain degree descent which was not remarkable. |
| Keywords: | FMD vaccine model animal virulence detection freeze-drying tests |
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