产蛋鸡卵黄性腹膜炎病原分离鉴定及防治试验

从25个鸡场疑似卵黄性腹膜炎的病料中分离到了18株大肠杆菌和2株沙门氏菌，经大肠杆菌O血清型鉴定，用优势血清型菌株分别接种改良的血清-葡萄糖-肉汤培养基所研制的多价油乳剂灭活苗，在开产前接种鸡群预防产蛋鸡卵黄性腹膜炎，预防效果良好，而采取多价油乳剂灭活苗预防接种配合活菌制剂益生素的使用，预防效果更佳。使用优势血清型菌株筛选的敏感药物硫酸丁胺卡那霉素、强力霉素同时配合清瘟败毒散和VC对卵黄性腹膜炎鸡群进行治疗，治疗效果良好。

Identification of the Yolk Peritonitis Pathogen from Layers and its Treatments

Eighteen strains of Escherichia coli(E.coli) and two of Salmonella were isolated from the suspected laryer specimen of yolk perionaeum peritonitis.With the O-serological typing assay,an inactivated oil-emulsion vaccine was produced by culturing the dominant E.coli strains in a modified serum-glucose-broth.The layer flocks immunized with the oil-emulsion vaccine before laying eggs were prevented efficiently from the yolk perionaeum peritonitis.Moreover,the vaccination combining with probiotics feeding induced more efficiently protective immunoreaction.The administration of such sensitive antibiotic as amikacin sulfate and dozycycline hyclate, which were selected for the dominant E.coli strains by the drug susceptibility test,combing with the Chinese traditional medicine and vitamin C was shown more effective for treatment of the yolk perionaeum peritonitis.