Evaluation of the mineralizable organic nitrogen in soil using an immunological technique |
| |
| Authors: | Shingo Matsumoto Noriharu Ae Yohichi Koyama Katsumasa Iijima Rumiko Kodashima Mari Hirata Junko Kasuga Nobuhisa Koga |
| |
| Institution: | (1) Education and Research Center for Biological Resources, Shimane University, Matsue, Shimane, Japan;(2) Biological and Environmental Science, Kobe University, Nada, Kobe, Japan;(3) Nippi Research Institute of Biomatrix, Senju-Midoricho, Adachi, Tokyo, Japan;(4) Iwate Agricultural Research Center, Kitakami, Iwate, Japan;(5) Hokkaido National Institute of Agriculture, Memuro, Kasai, Hokkaido, Japan |
| |
| Abstract: | Phosphate buffer-extractable organic nitrogen (PEON), which can be extracted from soil with a 67-mM neutral phosphate buffer
solution, is considered to be a major source of mineralizable nitrogen (N) that is ubiquitous in soil. We have developed a
Western blot analysis and enzyme-linked immune sorbent assay (ELISA) method for determining PEON. First, anti-PEON antibody
(IgG) from a rabbit was produced using purified PEON from a volcanic ash soil. PEON was detected as a ladder-like staining
pattern in the high-molecular-weight region and as a single band under 14 kDa in Western blot analysis using the anti-PEON
IgG. In the ELISA assay, the anti-PEON IgG could specifically react with PEON irrespective of soil type and management. Furthermore,
the absorbance at 450 nm in ELISA was highly correlated to the concentration of organic N (Norg) in phosphate extracts of different soils. We propose the immune assay using the anti-PEON IgG as a possible novel method
for evaluating available N in soil. |
| |
| Keywords: | ELISA Mineralizable nitrogen Phosphate buffer-extractable organic nitrogen (PEON) Western blot |
| 本文献已被 SpringerLink 等数据库收录！ |
|
