甜荞花同源异型基因FeMADS1的克隆和序列结构分析

采用同源克隆方法,并结合RACE技术,从甜荞花芽分离得到1个A类MADS-box基因FeMADS1的cDNA全长,GenBank登录号为KM386627,其cDNA全长1 107bp,包括1个编码234个氨基酸、长为705bp的开放阅读框。序列同源比对和分子系统发生分析表明,其蛋白与拟南芥AGL8(FUL)的相似性最高,属A类MADS-box基因亚家族中的euFUL进化系,含MADS、I、K和C末端4个明显的结构域,并且K结构域包含K1、K2和K3共3个保守的富含疏水氨基酸残基的亚结构域,C末端结构域含FUL型基因2个特有的模体:FUL motif和paleoAP1motfi。

Cloning and Sequence Analysis of FeMADS1 Gene from Fagopyrum esculentum

The cDNAs of an A -class MADS-box gene, FeMADS1, was cloned by homologous cloning and RACE method based on the sequence database of NCBI. The full length of FeMADS1 cDNA was 1 107 bp, which included an open reading frame (ORF) of 705 bp and coding for a polypeptide of 234 amino acid residues. Conceptual translation revealed that FeMADS1 protein had four domains: MADS, I, K and C-terminal domain . The C terminal region harbored two highly conserved FUL motif and paleoAP1 motif. Three highly conserved regions contained expected hydrophobic amino acids in the K-domain, namely subdomains K1, K2 and K3.