一种牛源扇头蜱及其携带梨形虫种类鉴定

为了鉴定从汉中市牛体表采集到的蜱种类及其携带病原梨形虫(Piroplasma)的种类,在形态学初步观察的基础上,用PCR技术基于线粒体16SrDNA对蜱种类进行了分子鉴定,并基于梨形虫18S rRNA基因分别检测蜱体内携带巴贝斯虫属(Babesia)、泰勒虫属(Theileria)等病原情况.结果显示,所采集到的67只...

Species Identification of Rhipicephalus on Cattle and Piroplosma Carried

In order to identify the species of ticks collected on cattle in Hanzhong City and the species of Piroplasma pathogens carried,the PCR detection technology was employed for the molecular identification of species of ticks based on mitochondrial 16S rDNA after the preliminary observation of morphology.On the basis of Piroplasma 18S rRNA gene,pathogens in ticks such as Babesia and Theileria were also detected.The results showed that all the 67 ticks collected were Rhipicephalus microplus,35 of which carried Piroplasma whose positive rate was 52.2%.The main Piroplasma pathogens carried include Babesia bovis,Babesia bigemina and Theileria orientails with the positive rates at 1.5%,3%and 47.7%respectively.The study showed that the dominant species of ticks on cattle in Hanzhong area is R.microplus,which can carry various Piroplasma pathogens such as B.bovis,B.bigemina,and T.orientalis,etc.It is suggested that the local government should guard against tick-borne piroplasmosis and inhibit the risk of tick borne disease transmission.