| 山羊流产衣原体ompA和CPAF基因的序列分析及原核表达 |
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| 引用本文： | 徐海玲,杨萍,张金生,王满定,刘广阔,李梦磊,张琪,许信刚.山羊流产衣原体ompA和CPAF基因的序列分析及原核表达[J].动物医学进展,2022,43(2):1-6. |
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| 作者姓名： | 徐海玲 杨萍 张金生 王满定 刘广阔 李梦磊 张琪 许信刚 |
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| 作者单位： | 西北农林科技大学动物医学院,陕西杨凌712100,咸阳市动物疫病预防控制中心,陕西咸阳712000,甘肃元生农牧科技有限公司,甘肃永昌737200 |
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| 基金项目： | 省级农业专项资金支持科技创新驱动项目 |
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| 摘 要： | 为了解山羊流产衣原体陕西分离株ompA和CPAF基因的遗传变异情况及制备检测抗原,根据GenBank收录的ompA和CPAF基因序列设计合成特异性引物,分别进行ompA基因和CPAF基因的基因克隆、序列分析及原核表达.结果表明,成功克隆出大小为1 053 bp的ompA基因和大小为1 056 bp的CPAF基因;核酸序...
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| 关 键 词： | 山羊流产衣原体 ompA基因 CPAF基因 序列分析 原核表达 |
Sequence Analysis and Prokaryotic Expression of ompA and CPAF Genes in Chlamydophila abortus of Goats |
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| XU Hai-ling,YANG Ping,ZHANG Jin-sheng,WANG Man-ding,LIU Guang-kuo,LI Meng-lei,ZHANG Qi,XU Xin-gang.Sequence Analysis and Prokaryotic Expression of ompA and CPAF Genes in Chlamydophila abortus of Goats[J].Progress In Veterinary Medicine,2022,43(2):1-6. |
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| Authors: | XU Hai-ling YANG Ping ZHANG Jin-sheng WANG Man-ding LIU Guang-kuo LI Meng-lei ZHANG Qi XU Xin-gang |
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| Institution: | (College of Veterinary Medicine,Northwest A&F University,Yangling,Shaanxi,712100,China;Xianyang Animal Disease Prevention and Control Center,Xianyang,Shaanxi,712000,China;Gansu Yuansheng Agriculture and Animal Husbandry Technology Co.LTD,Yongchang,Gansu,737200,China) |
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| Abstract: | In order to understand the genetic variation of the ompA and CPAF genes of Chlamydophila abortus and prepare the detection antigens,specific primers were designed and synthesized according to the sequences of the ompA and CPAF genes in GenBank.The gene cloning,sequence analysis and prokaryotic expression of the ompA and CPAF genes were carried out respectively.The results showed that the ompA gene with a size of 1053 bp and the CPAF gene with a size of 1056 bp were successfully cloned.Nucleic acid sequence analysis showed that the homologies of ompA and CPAF genes were 99.54%-99.91%and 91%-100%,respectively,with the reported ompA and CPAF genes of C.abortus.Prokaryotic expression strains of ompA and CPAF genes were successfully constructed.After IPTG induction,38.6 ku and 38.7 ku OmpA and CPAF recombinant proteins were expressed,respectively.Western blot showed that the purified and refolded recombinant protein can be combined with goat C.abortus positive serum.The results of this experiment laid the foundation for the further establishment of rapid antibody ELISA detection methods. |
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| Keywords: | Chlamydophila abortus of goat ompA gene CPAF gene sequence analysis prokaryotic expression |
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