| 新疆南疆部分地方品种羊无浆体的分子检测与鉴定 |
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| 引用本文： | 金紫薇,罗慧丽,石文玉,辛园园,郎平,薛芙蓉,陶大勇,赵爱云,齐萌.新疆南疆部分地方品种羊无浆体的分子检测与鉴定[J].动物医学进展,2022,43(2):59-63. |
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| 作者姓名： | 金紫薇 罗慧丽 石文玉 辛园园 郎平 薛芙蓉 陶大勇 赵爱云 齐萌 |
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| 作者单位： | 塔里木大学动物科学学院新疆生产建设兵团塔里木动物疫病诊断与防控工程实验室,新疆阿拉尔843300 |
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| 基金项目： | 新疆生产建设兵团中青年科技创新领军人才计划项目;新疆生产建设兵团重点领域科技攻关计划项目;塔里木大学大学生创新创业训练计划项目 |
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| 摘 要： | 为了解新疆南疆部分地方品种羊的无浆体感染情况和分子特征,用PCR法检测新疆南疆5种地方品种绵羊共100份血液DNA样本,发现无浆体总感染率为67.0％(67/100).以多浪羊感染率最高,为100％(20/20),和田羊感染率最低,为44.0％(11/25);散养和圈养羊的无浆体感染率分别为74.0％(37/50)和6...
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| 关 键 词： | 无浆体 检测 鉴定 绵羊 |
Molecular Detection and Identification of Anaplasma spp.in Some Local Breeds of Sheep in Southern Xinjiang |
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| JIN Zi-wei,LUO Hui-li,SHI Wen-yu,XIN Yuan-yuan,LANG Ping,XUE Fu-rong,TAO Da-yong,ZHAO Ai-yun,QI Meng.Molecular Detection and Identification of Anaplasma spp.in Some Local Breeds of Sheep in Southern Xinjiang[J].Progress In Veterinary Medicine,2022,43(2):59-63. |
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| Authors: | JIN Zi-wei LUO Hui-li SHI Wen-yu XIN Yuan-yuan LANG Ping XUE Fu-rong TAO Da-yong ZHAO Ai-yun QI Meng |
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| Institution: | (College of Animal Science,Tarim University/Engineering Laboratory of Tarim Animal Diseases Diagnosis and Control,Xinjiang Production&Construction Corps,Alar,Xinjiang,843300,China) |
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| Abstract: | In order to understand the infection and molecular characteristics of Anaplasma spp.in some local breeds of sheep in Southern Xinjiang,a total of 100 blood DNA samples from five local breeds of sheep in Southern Xinjiang were detected by PCR,and the total infection rate of Anaplasma spp.was 67.0%(67/100).The highest infection rate of Anaplasma spp.was 100%(20/20)in Duolang sheep,while the lowest infection rate was 44.0%(11/25)in Hotan sheep.The infection rates of Anaplasma spp.in grazing and captive sheep were 74.0%(37/50)and 60.0%(30/50),respectively.Among 67 positive samples of Anaplasma spp.,65 positive samples were detected as A.ovis and 14 positive samples were detected as A.phagocytophilum,and 12 positive samples were A.ovis and A.phagocytophilum mixed infection.No A.bovis and A.capra were detected.Sequence analysis showed three genotypes in 65 A.ovis,including genotype AO1(n=40),genotype AO2(n=12)and genotype AO3(n=13);and three genotypes in 14 A.phagocytophilum,including genotype AP1(n=12),genotype Ap2(n=1)and genotype AP3(n=1).The results showed that the infection of Anaplasma spp.was common in sheep in Southern Xinjiang,and its detection and control should be strengthened. |
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| Keywords: | Anaplasma detection identification sheep |
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