基于SSR标记的雪茄烟种质资源指纹图谱库的构建及遗传多样性分析

为从分子水平研究我国雪茄烟种质资源的遗传多样性差异并建立雪茄烟品种的DNA指纹图谱数据库,本研究利用43对多态性好的SSR引物对220份雪茄烟种质进行遗传多样性分析,筛选出14对核心引物对雪茄烟种质进行指纹图谱的构建。结果表明,43对SSR引物在220份雪茄烟种质材料中共扩增出243个等位基因,平均每个标记5.65个,变幅为2～13,每个位点的多态性信息量(polymorphism information content,PIC)变化为0.2078～0.9087,平均为0.6360。有效等位基因数(number of effective alleles, Ne)范围为1.3081～11.7876,平均有效等位基因数为3.9077;观测杂合度(observed heterozygosity, H_o)变化范围为0.0828～0.7639,平均为0.3191;预期杂合度(expected heterozygosity,He)的变化范围为0.2361～0.9172,平均为0.6809;种群平均Shannon遗传多样性指数(Shannon genetic diversity...

Construction of SSR fingerprint database and genetic diversity analysis of cigar germplasm resources

In order to investigate the genetic diversity of Chinese cigar germplasm resources in molecular level and to establish the DNA fingerprint database of cigar varieties,43 pairs of SSR primers with good polymorphic were used to analyze the genetic diversity of 220 cigar germplasms,and 14 pairs of core primers were screened from 43 pairs of SSR primers to construct the fingerprint database of 220 cigar germplasms in the study.Results showed that a total of 243 alleles were amplified by 43 pairs of SSR primers in 220 cigar germplasm materials,with an average of 5.65 per marker,ranging from 2 to 13.The polymorphic information content(PIC)of each locus varied from 0.2078 to 0.9087 with an average of 0.6360.The number of effective alleles(N_e)ranged from 1.3081 to 11.7876,and the average number of effective alleles was 3.9077.The observed heterozygosity(H_o)ranges from 0.0828 to 0.7639 with an average of 0.3191.The expected heterozygosity(H_e)ranged from 0.2361 to 0.9172 with an average of 0.6809.The average Shannon genetic diversity index(I)was 1.3756,the genetic distance was between 0.0233 and 0.9286,and the average genetic distance was 0.6816.Unweighted pair-group method with arithmetic means(UPGMA)cluster analysis showed that the tested cigar resources can be divided into three groups at the genetic distance of 0.74.All tested materials were divided into two groups by population genetic structure analysis and principal component analysis(PCA).Based on the primer analysis and phenotypic identification,it was determined that the‘LZ’,‘FS’and‘MED’,‘SDDY’and‘MDJ 05-1’,‘Florida 513’and‘CA0701’were the same species with different names.One variety retained one germplasm,leaving 216 different germplasm.14 pairs of SSR primers which could distinguish all the tested materials were screened to construct the fingerprint database of 216 cigar cultivars from 43 pairs of SSR primers as core primers.In conclusion,cigar germplasm resources in China had a high level of genetic diversity.In this study,the constructed SSR fingerprint database and genetic analysis of cigar germplasm resources provided the scientific basis for the screening and identification of high-quality cigar germplasms resources,the mining of important genes,and the broadening the basis of cigar genetic breeding.