利用东乡普通野生稻染色体片段置换系定位产量相关性状QTL

以东乡普通野生稻和日本晴为亲本构建的染色体片段置换系为研究材料, 2019年分别在北京、山东临沂和江西南昌对分蘖数、穗粒数和粒形等11个产量相关性状进行多环境鉴定,结合染色体片段置换系基因型数据定位水稻产量相关性状QTL。3个环境共检测到68个QTL,包括株高4个、穗长5个、分蘖数2个、一次枝梗数7个、一次枝梗粒数8个、二次枝梗数8个、二次枝梗粒数10个、每穗粒数6个、千粒重7个、粒长8个和粒宽3个; LOD值介于2.50～12.66之间,贡献率变幅为4.67%～27.79%,15个QTL的贡献率大于15%;24个QTL与已报道位点/基因位置重叠,44个QTL为新发现位点; 6个QTL在2个环境能被检测到, 1个QTL qTGW2能在3个环境检测到,且是还未报道的新位点。最后,利用BSA法验证了qPH7、qPBPP8-2和qGW10三个QTL的可靠性。本研究将为后续产量相关性状基因克隆以及进一步解析其遗传基础和分子调控机制奠定基础。

Mapping QTLs for yield-related traits using chromosome segment substitution lines of Dongxiang common wild rice(Oryza rufipogon Griff.)and Nipponbare(Oryza sativa L.)

In former study,we constructed a chromosome segment substitution lines(CSSLs)of Dongxiang common wild rice(Oryza rufipogon Griff.)in the background of Nipponbare(Oryza sativa L.).In this study,in order to investigate 11 yield-related traits,such as tillering number,grains per panicle and grain shapes,the CSSLs were planted in Beijing,Linyi and Nanchang.The results of quantitative trait loci(QTLs)for yield-related traits showed that a total of 68 QTLs were detected,including 4 QTLs for plant height,5 QTLs for panicle length,2 QTLs for tillering number,7 QTLs for primary branch grain number,8 QTLs for primary branch grain number,8 QTLs for secondary branch grain number,10 QTLs for secondary branch grain number,6 QTLs for grains per panicle,7 QTLs for 1000-grain weight,8 QTLs for grain length and 3 QTLs for grain width.LOD score of the detected QTLs ranged from 2.50 to 12.66.The phenotypic variation explained by these QTLs ranged from 4.67%to 27.79%.There were 15 QTLs with a contribution rate more than 15%,24 QTLs overlapped with the reported loci or gene position,44 QTLs newly detected loci.In addition,6 QTLs were stably detected at two sites,and 1 QTL(qTGW2)as a novel QTL was detected at three sites.Finally,the reliability of the three QTLs of qPH7,qPBPP8-2 and qGW10 was verified by BSA.Our results will be helpful for the subsequent cloning of yield-related trait genes and further analysis of their genetic basis and molecular regulation mechanism.