| 金华猪和大约克猪分娩后21d乳腺microRNAs的预测和差异研究 |
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| 引用本文： | 党文庆,张立凡,徐宁迎,王颖,蔡兆伟,翟继鹏,吕尊周,郝柱.金华猪和大约克猪分娩后21d乳腺microRNAs的预测和差异研究[J].农业生物技术学报,2011,19(5). |
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| 作者姓名： | 党文庆 张立凡 徐宁迎 王颖 蔡兆伟 翟继鹏 吕尊周 郝柱 |
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| 作者单位： | 浙江大学动物科学学院,杭州,310029 |
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| 基金项目： | 国家转基因生物新品种培育重大专项(No.2009ZX08006-009B); 国家自然科学基金(No.31000997)共同资助 |
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| 摘 要： | microRNAs(miRNAs)是一类单链小RNAs,是重要的基因表达调控因子,通过与靶基因的3'UTR序列互补来调控基因的表达。鉴定乳腺组织的miRNAs及其靶基因,对揭示miRNA在乳腺组织基因调控中的作用十分重要。本研究分别从分娩后21d金华和大约克母猪的乳腺组织中提取小RNA并构建各自的cDNA文库,通过Illumina Genome AnalyzerⅡx测序平台对它们进行高通量深度测序。将测序初始序列经过序列类型、长度、丰度的筛选得到比对序列,然后与已有的miRNA数据库、Genome和EST数据库进行比对。将两个猪种检测到的miRNA进行品种间差异性比较,并在差异性结果中选择相对丰度较高的前10个miRNA对其进行生物信息学分析。结果发现:金华猪乳腺组织的比对序列有9672024条,大约克猪6946905条;共检测到金华猪单一序列miRNA有848个,大约克猪683个;金华猪预测的新单一序列miRNA有369个,大约克猪231个;两个猪种间差异显著的miRNA有288个(P<0.01);相对丰度较高的前10个miRNA的靶基因有1829个,这些靶基因主要参与了抗原加工和呈递、Ⅰ型糖尿病、缝隙连接...
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| 关 键 词： | miRNA 猪 乳腺 测序 表达差异 |
Different Expressing of microRNAs in Mammary Gland Tissue of Jinhua and Yorkshire Pig Breeds on Postpartum 21 Days |
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| Dang Wenqing,Zhang Lifan,Xu Ningying,Wang Ying,Cai Zhaowei,Zhai Jipeng,Lv Zunzhou,Hao Zhu.Different Expressing of microRNAs in Mammary Gland Tissue of Jinhua and Yorkshire Pig Breeds on Postpartum 21 Days[J].Journal of Agricultural Biotechnology,2011,19(5). |
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| Authors: | Dang Wenqing Zhang Lifan Xu Ningying Wang Ying Cai Zhaowei Zhai Jipeng Lv Zunzhou Hao Zhu |
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| Institution: | Dang Wenqing Zhang Lifan Xu Ningying* Wang Ying Cai Zhaowei Zhai Jipeng Lv Zunzhou Hao Zhu College of Animal Science,Zhejiang University,Hangzhou 310029,China |
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| Abstract: | microRNAs(miRNAs) is a class of single-strand small RNAs that regulate gene expression by binding to complementary sequences in the 3' untranslated region(3'UTR) of target mRNAs. In a sense, identification of miRNAs and their targets in mammary gland tissue is essential to proclaim the function of miRNAs on gene expression and regulation. In the present study, small RNAs were extracted from porcine mammary gland tissue of Jinhua and Yorkshire breed respectively and used to construct a cDNA library. Then it ... |
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| Keywords: | miRNA Pig Mammary gland Sequencing Different expression |
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