| 利用三重测交群体剖析玉米苗期性状杂种优势的遗传学基础 |
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| 引用本文： | 宋方威,彭惠茹,张义荣,杨小红,刘婷,孙其信,倪中福. 利用三重测交群体剖析玉米苗期性状杂种优势的遗传学基础[J]. 农业生物技术学报, 2011, 19(5). DOI: 10.3969/j.issn.1674-7968.2011.05.001 |
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| 作者姓名： | 宋方威 彭惠茹 张义荣 杨小红 刘婷 孙其信 倪中福 |
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| 作者单位： | 1. 农业生物技术国家重点实验室/中国农业大学杂种优势研究与利用教育部重点实验室/作物基因组与遗传改良农业部重点实验室/作物遗传改良北京市重点实验室,北京100193;国家植物基因研究中心,北京100193
2. 国家玉米改良中心,北京,100193 |
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| 基金项目： | 国家重点基础研究发展计划(973)项目(No.2007CB109000); 国家杰出青年科学基金(No.30925023); 国家自然科学基金(No.30671297,No.30771342)共同资助 |
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| 摘 要： | 为了探讨玉米苗期性状及其杂种优势形成的遗传学基础,以强优势玉米(Zea maysL.)杂交种组合豫玉22及其重组近交系为基础材料,采用三重测交(triple testcross,TTC)遗传交配设计,组配了包含312个测交后代的TTC群体,通过复合区间作图法检测到了30个控制发芽后第4天的最长根长、苗高、初生根数、根干重及叶干重的QTLs,并且在第2、3和7染色体上存在4个同时控制不同苗期性状的QTL区域。分析发现,在利用Z1和Z2数据定位出的22个QTLs中,以超显性位点最多(11个),加性(5个)和部分显性较少(5个),而显性最少(1个)。另外,还检测到8个QTLs与遗传背景之间的互作和16对不同标记间的互作。据此,我们提出超显性和上位性是玉米苗期性状及其杂种优势形成的主要遗传学基础。关键词玉米,苗期性状,三重测交,杂种优势,QTL定位
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| 关 键 词： | 玉米 苗期性状 三重测交 杂种优势 QTL定位 |
Heterosis for Seedling Traits in Maize(Zea mays L.) revealed by Quantitative Trait Loci Analysis of the Triple Testcross Design |
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| Song Fangwei,Peng Huiru,Zhang Yirong,Yang Xiaohong,Liu Ting,Sun Qixin,Ni Zhongfu. Heterosis for Seedling Traits in Maize(Zea mays L.) revealed by Quantitative Trait Loci Analysis of the Triple Testcross Design[J]. Journal of Agricultural Biotechnology, 2011, 19(5). DOI: 10.3969/j.issn.1674-7968.2011.05.001 |
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| Authors: | Song Fangwei Peng Huiru Zhang Yirong Yang Xiaohong Liu Ting Sun Qixin Ni Zhongfu |
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| Affiliation: | Song Fangwei1,2 Peng Huiru1,2 Zhang Yirong3 Yang Xiaohong3 Liu Ting1,2 Sun Qixin1,2 Ni Zhongfu1,2* 1 State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Heterosis and Utilization,Ministry of Education/Key Laboratory of Crop Genomics and Genetic Improvement,Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement,China Agricultural University,Beijing 100193,China,2 National Plant Gene Research Centre (Beijing),3 National Maize Improvement Center o... |
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| Abstract: | To investigate the genetic basis of heterosis in maize seedling traits, highly heterotic maize(Zea mays L.) hybrid Yuyu22 and RILs(recombinant inbred lines) were used as basic population to construct TTC(triple testcross) population, which included 312 testcross progenies by using TTC genetic mating design. Thirty QTLs were detected for the longest root length(LRL), shoot height(SH), primary root number(RN), root dry matter weight(RDW) and shoot dry matter weight(LDW) by using composite interval mapping. An... |
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| Keywords: | Maize Seedling traits Triple testcross Heterosis QTL |
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